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《Journal of The Fourth Military Medical University》 2005-11
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Fluorescence detection of propofol drug concentration in plasma

LU Li-Hua1,ZHANG Hui2,GAO Rong1,WEI Lin-Lin1,WU Hong1, LI Xiao-Ye11Department of Chemistry, School of Pharmacy, 2Department of Anesthesiology, Stomatological Hospital, Fourth Military Medical University, Xi'an 710033, China  
AIM: To study the use of fluorescence detection in determining the propofol drug concentration in plasma. METHODS: Buffer solution of phosphoric acid and cyclohexane-tertbutanol were added to plasma samples with different propofol concentration. After being vibrated, the samples were centrifugated. The upper-clear solution was taken to determine the fluorescence intensity. The fluorescence detective excitation waves and emission waves were 265 nm and 310 nm respectively. RESULTS: Between the propofol concentrations and the fluorescence intensities, there was a good linear relationship in the range of 0.1-15.0 mg/L (r=0.9997). The average recovery was more than 95%. The relative standard deviation (RSD) of intra-day and inter-day assays were all less than 3%. CONCLUSION: The method is sample, accurate and sensitive for determining drug concentration of propofol in plasma.
【Fund】: 国家自然科学基金(30300456)
【CateGory Index】: R96
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【References】
Chinese Journal Full-text Database 1 Hits
1 LONG You-qi,HE Dong-ping,WAN Jing-yuan,YANG Jun-qing and SHANG Jing-chuan(Department of Pharmaceutical,Chongqing Medical University,Chongqing 400016);Determination of the plasma protein binding rate of propofol microemulsion injection in plasma of beagel[J];Chinese Journal of Analysis Laboratory;2013-03
【Citations】
Chinese Journal Full-text Database 1 Hits
1 CHAI Wei, YU Dai Hua, GAO Chang Jun, YANG Yong Hui, SUN Xu De Department of Anesthesiology, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China;Target-controlled infusion with propofol for total intravenous anesthesia[J];Journal of The Fourth Military Medical University;2002-15
【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 HE Sai Lin 1, ZHANG Ying Min 1, YUE Yun 2, CHEN Xiao Guang 1, GUO Hui 1, LIU Su 1 1Department of Anesthesiology, 304th Hospital of Chinese PLA, Beijing 100037, China, 2Department of Anesthesiology, Chaoyang Hospital, Capital Medical Science University, Beijing 100021, China;Effects of propofol anesthesia on noradrenaline release in rat hippocampus[J];Journal of The Fourth Military Medical University;2004-15
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1 WANG Hui-xia, XU Li-xian, MA Jia-hai,et al(Department of Anesthesiology, Stomatological College,Fourth Military Medical University, Xi'an710032,China);Blood concentration determination of propofol in mice with spectrofluorometry[J];Anhui Medical and Pharmaceutical Journal;2005-08
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【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 LONG You-qi,HE Dong-ping,WAN Jing-yuan,YANG Jun-qing and SHANG Jing-chuan(Department of Pharmaceutical,Chongqing Medical University,Chongqing 400016);Determination of the plasma protein binding rate of propofol microemulsion injection in plasma of beagel[J];Chinese Journal of Analysis Laboratory;2013-03
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