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《Journal of The Fourth Military Medical University》 2005-11
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Fluorescence detection of propofol drug concentration in plasma

LU Li-Hua1,ZHANG Hui2,GAO Rong1,WEI Lin-Lin1,WU Hong1, LI Xiao-Ye11Department of Chemistry, School of Pharmacy, 2Department of Anesthesiology, Stomatological Hospital, Fourth Military Medical University, Xi'an 710033, China  
AIM: To study the use of fluorescence detection in determining the propofol drug concentration in plasma. METHODS: Buffer solution of phosphoric acid and cyclohexane-tertbutanol were added to plasma samples with different propofol concentration. After being vibrated, the samples were centrifugated. The upper-clear solution was taken to determine the fluorescence intensity. The fluorescence detective excitation waves and emission waves were 265 nm and 310 nm respectively. RESULTS: Between the propofol concentrations and the fluorescence intensities, there was a good linear relationship in the range of 0.1-15.0 mg/L (r=0.9997). The average recovery was more than 95%. The relative standard deviation (RSD) of intra-day and inter-day assays were all less than 3%. CONCLUSION: The method is sample, accurate and sensitive for determining drug concentration of propofol in plasma.
【Fund】: 国家自然科学基金(30300456)
【CateGory Index】: R96
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3 HUANG Zhuo WANG Qi WANG Jing-yu LI Qun WU Xin-min JIANG Jian-yu (Department of Central Instrument, School of Public Health, Peking University, Beijing 100083) (Department of Anesthesiology, Third Hospital of Peking University, Beijing 100083);RP-HPLC Method for Determination of Propofol in Human Serum[J];The Chinese Journal of Clinical Pharmacology;2003-04
4 Liu Peizhong, Jia Baosen, Zhang Hong. Department of Anesthesiology, General Hospital of Peoples Liberation Army, Beijing 100853, China;Comparison of the effects of propofol total intravenous anesthesia vs higher dosages of fentanyl anesthesia on myocardial function in CABG operation[J];Medical Journal of Chinese People's Liberation Army;2005-12
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【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 LONG You-qi,HE Dong-ping,WAN Jing-yuan,YANG Jun-qing and SHANG Jing-chuan(Department of Pharmaceutical,Chongqing Medical University,Chongqing 400016);Determination of the plasma protein binding rate of propofol microemulsion injection in plasma of beagel[J];Chinese Journal of Analysis Laboratory;2013-03
2 Song Lin;Hong Zhanying;Zhang Runsheng;Liang Chen;Chen Yongsheng;Chai Yifeng;Fan Guorong;Fujian University of Traditional Chinese Medicine;School of Pharmacy,Second Military Medical University,Shanghai Key Laboratory for Pharmaceutical Metabolites Research;Shanghai Institiute of Forensic Science,Shanghai Key Laboratory of Crime Scene Evidence;;Determination of propofol in human blood by GPC-GC/MS[J];Chinese Journal of Forensic Medicine;2015-02
3 LI Li;LI Pei;Pharmacy College,Xinjiang Medical University;;Determination of propofol in human plasma by RP- HPLC gradient elution[J];Anhui Medical and Pharmaceutical Journal;2015-04
4 REN Qiu-xia;LIU Miao;WU Rong-rong;QIN Li;ZHU Hong;CAO Ming-xue;LIU Feng-qun;Department of Pharmacy,Hospital 302 of PLA;;Determination of Propofol Content in Human Plasma by UPLC[J];Pharmaceutical Journal of Chinese People's Liberation Army;2014-06
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