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《Journal of The Fourth Military Medical University》 2005-14
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Effects of arsenious acid on proliferation and PCNA, c-Myc protein expression of hepatocarcinoma cells

ZHANG Chuan-Hai 1, XU Ge-Liang 1, GE Yong-Sheng 1, ZHAI Zhi-Min 2, YU Ji-Hai 1 1Department of General Surgery, 2Department of Central Laboratory, Anhui Provincial Hospital, Hefei 230001, China  
AIM: To investigate the effect of arsenious acid on the proliferation as well as the PCNA, c-Myc protein expression of HCC cell line BEL-7402. METHODS: The inhibitive effect of arsenious acid on BEL-7402 was measured with MTT and TD and the colony-forming rate of BEL-7402 cells was studied by colony-forming assay. The expression of PCNA and c-Myc protein was determined by immunohistochemical analysis. RESULTS: After treatment with arsenious acid, the inhibition rate of BEL-7402 cells increased (2.0, 4.0, 8.0μmol/L arsenious acid treatment for 24, 48, 72 h; the inhibition rate was 27.13%, 42.65%, 47.74%; 35.52%, 53.24%, 60.41%;50.61%, 65.88%, 70.81% respectively; P0.05 vs control), TD delayed gradually (2.0, 4.0, 8.0 μmol/L arsenious acid treatment for 48 h; TD was 24.68±3.99; 32.42±4.46; 36.95±8.91 respectively; P0.05 and P0.01 vs control) while the colony-forming rate decreased (0, 8.0 μmol/L arsenious acid treatment for 48 h; the colony-forming rate was 46.00%, 18.80% respectively; P0.05). In arsenious acid treated BEL-7402 cells, PCNA and c-Myc expressions were significantly down-regulated at protein level. CONCLUSION: Arsenious acid can inhibit the proliferation of BEL-7402 cells, possibly by down-regulating the PCNA and c-Myc protein.
【CateGory Index】: R735.7
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【References】
Chinese Journal Full-text Database 3 Hits
1 ZHANG Xiao-Zhan~1, NAN Ke-Jun~2, LI Chun-Li~2,CHEN Ling~2 ~1Department of Oncology, Fifth Affiliated Hospital, Zunyi Medical College, Zhuhai 519100, China, ~2Department of Oncology, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an 710061, China;Expression of p34~(cdc2) in hepatocellular carcinoma and its relationship with cell proliferation and apoptosis[J];Journal of the Fourth Military Medical University;2006-21
2 HUANG Xue-wu1,LI Yi-qun2 1.The First Affiliated Hospital of Traditional Chinese Medicine,Guangzhou,China,510405; 2.Huizhou Central People's Hospital,Huizhou,Guangdong,China,516001;Current Status on Resisting Primary Hepatic Carcinoma With Arsenous acid Injection[J];Guiding Journal of Traditional Chinese Medicine and Pharmacy;2008-06
3 YIN Mei-zhen1,2,LI Shi-pu2,HAN Ying-chao2(1.School of Medicine,Huangshi Institute of Technology,Huangshi 435003,China;2.Biomedical Material and Engeneering Center,Wuhan University of Technology,Wuhan 430070,China);Effect of Hydroxyapatite Nanoparticles on the PCNA Expression of Hepatoma Carcinoma Cells[J];Journal of Wuhan University of Technology;2007-08
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Yun Jingping1  Lin Hanliang2  Wu Qiuliang1, et al Sun Yat-sen University of Medical sciences,Guangzhou 5100891 Dept.of Pathology of Cancer Hospital,Guangzhou 5100602 Dept.of Pathology  ;Clinicopathologic immunohistochemical and ultrastructural observation on small cell carcinomas of the esophagus[J];CHINESE JOURNAL OF CANCER;1998-04
2 Li Yongjian et al ( Shanghai University of TCM );Clinical Epidemiological Investigation and Research of Syndrome Distribution Law in Chinese Medicine of 2060 Cases of Primary Liver Carcinoma[J];China Journal of Traditional Chinese Medicine and Pharmacy;2003-03
3 HU Bing, AN Hong mei, LI Xin min (胡兵,等) The First people Hospital of Chenzhou (Chenzhou,423000,China);The Study of Chinese Medicine Compound/DPC Induced Differentiation on Human Heptocarcinoma Cell Lines HCC 9204 and It's Mechanism[J];JOURNAL OF CHENGDU UNVERSITY OF TARDITIONAL CHINESE MEDICINE;2000-01
4 ZHANG Xia, WANG Pi-long, TAO Xiao-hong(Department of Gastroenterology, the First Affiliated Hospital,Chongqing University of Medical Sciences, Chongqing 400016,China);Expression of cell cycle and associated genes of hepatomacellular carcinoma by arsenic trioxide[J];Chongqing Medical Journal;2004-11
5 WANG Ning~1,ZHU Yan-ming~2,PIAO Ming-yu~3 MENG Dan~1(1.College of Urban and Environmental Sciences of Northeast Normal University,Changchun,Jilin 130024; 2.Northeast Institute of Geography and Agricultural Ecology,Chinese Academy of Sciences,Changchun,Jilin 130012;3.Research Institute for Bioresource,Okayama University,Japan 700-0046);Chemical Ecology Effect of Mercury Pollution on Upper Songhua River Areas[J];Scientia Geographica Sinica;2005-06
6 QIAN Yan 1, LING Chang-Quan 1, JIN Yan 2 1Department of Chinese Traditional Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China, 2Department of Pathology, Stomatological College, Fourth Military Medical Un;Effects of ganfujian granule on C-myc IGF-Ⅱ gene expression in rat hepatocarcinogenesis[J];Journal of The Fourth Military Medical University;2002-18
7 WANG Hong Guang 1, LI Kai Zong 1, DOU Ke Feng 1, CHEN Dan 2, WANG Chun Mei 2 1Department of Hepatobiliary Surgery, Xijing Hospital, 2Center of Electron Microscope, Faculty of Preclinical Medicine, Fourth Military Medical University,;Distribution and effect of antisense oligodeoxynucleotide in human hepatocellular carcinoma cell[J];Journal of The Fourth Military Medical University;2002-21
8 ZHENG Jian Yong 1, LI Kai Zong 2, WANG Wei Zhong 1 1Department of Gastrointestinal Surgery, 2Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an 710033, China;Relationship between p~(27)~(kip1) and cellular proliferation and apoptosis in hepatocellular carcinoma[J];Journal of The Fourth Military Medical University;2003-15
9 LI Xiao Ju 1, YAN Pei Song 2 1Department of Pathology, Hospital of Xian Red Cross Society, Xian 710054, China, 2Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xian 710033, China;Expression and significances of HSP70 and PCNA in serous carcinoma of human ovary[J];Journal of The Fourth Military Medical University;2003-16
10 ZHANG Xiao-Zhan, NAN Ke-Jun, RUAN Zhi-Ping, XU Rui, GUO Hui Department of Oncology, First Hospital, Xi'an Jiaotong University, Xi'an 710061, China;Clinical significance of p34~(cdc2) expression in hepatocellular carcinoma tissue[J];Journal of The Fourth Military Medical University;2005-01
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