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《Journal of The Fourth Military Medical University》 2005-14
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Effects of arsenious acid on proliferation and PCNA, c-Myc protein expression of hepatocarcinoma cells

ZHANG Chuan-Hai 1, XU Ge-Liang 1, GE Yong-Sheng 1, ZHAI Zhi-Min 2, YU Ji-Hai 1 1Department of General Surgery, 2Department of Central Laboratory, Anhui Provincial Hospital, Hefei 230001, China  
AIM: To investigate the effect of arsenious acid on the proliferation as well as the PCNA, c-Myc protein expression of HCC cell line BEL-7402. METHODS: The inhibitive effect of arsenious acid on BEL-7402 was measured with MTT and TD and the colony-forming rate of BEL-7402 cells was studied by colony-forming assay. The expression of PCNA and c-Myc protein was determined by immunohistochemical analysis. RESULTS: After treatment with arsenious acid, the inhibition rate of BEL-7402 cells increased (2.0, 4.0, 8.0μmol/L arsenious acid treatment for 24, 48, 72 h; the inhibition rate was 27.13%, 42.65%, 47.74%; 35.52%, 53.24%, 60.41%;50.61%, 65.88%, 70.81% respectively; P0.05 vs control), TD delayed gradually (2.0, 4.0, 8.0 μmol/L arsenious acid treatment for 48 h; TD was 24.68±3.99; 32.42±4.46; 36.95±8.91 respectively; P0.05 and P0.01 vs control) while the colony-forming rate decreased (0, 8.0 μmol/L arsenious acid treatment for 48 h; the colony-forming rate was 46.00%, 18.80% respectively; P0.05). In arsenious acid treated BEL-7402 cells, PCNA and c-Myc expressions were significantly down-regulated at protein level. CONCLUSION: Arsenious acid can inhibit the proliferation of BEL-7402 cells, possibly by down-regulating the PCNA and c-Myc protein.
【CateGory Index】: R735.7
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