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《Journal of The Fourth Military Medical University》 2005-21
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Effects of recombinant Caspase-3 on human laryngocarcinoma cell line Hep-2 through coexpression with GFP

YU Xiao-Lin, LIU Hong, CHEN Wan-Ping, CHENG Peng-YuanOutpatient Department, Fourth Military Medical University, Xi'an 710033, China  
AIM: To investigate the apoptosis induction effect of the expression of recombinant Caspase-3 on laryngocarcinoma cell line Hep-2. METHODS: Recombinant Caspase-3 gene was subcloned into the GFP reporting vector pEGFP-C1 to generate the expressing vector pEGFP-Caspase-3 by DNA recombinant technique. Human laryngocarcinoma cell line Hep-2 was transfected with Caspase-3 gene by Lipofectamine 2000 and the expression of recombinant Caspase-3 was observed by RT-PCR. The cell morphological changes were observed under fluorescent and (electronic) microscope and the cell survival rate after transfection was assayed by MTT method. RESULTS: Recombinant Caspase-3 gene was stably expressed in Hep-2. Recombinant Caspase-3 induced obvious apoptosis in vitro. The MTT results suggested that the cell curve declined after transfection with Caspase-3. CONCLUSION: Recombinant Caspase-3 can inhibit the growth of the laryngocarcinoma cell line Hep-2 and effectively induce it to apoptosis.
【CateGory Index】: R739.65
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