Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of The Fourth Military Medical University》 2005-22
Add to Favorite Get Latest Update

Relationship between cell apoptosis and Bcl-2/Bax ratio in neural cells after acute spinal cord injury in rats

MA Li-Jie~1,WANG Hong~2,WANG Xiang-Li~1,LIU Ying-Jie~1,WANG Ping~21Department of Orthopedics,Affiliated Hospital,~2Department of Foreign Language,North China Coal Medical College,Tangshan 06300,China  
AIM: To investigate the expressions of Bcl-2 and Bax and the apoptosis in the neural cells at different time points after acute spinal cord compression injury.METHODS: Twenty-eight rats were divided at random into 2 groups: control group(n=4) and injury group (n=24).The former underwent laminotomy without spinal cord compression,while the latter with spinal cord compression so as to establish the rat models with acute spinal cord injury.The injury group was subdivided randomly into 6 groups,4 in each group.The spinal cord in the injury group was taken at 4 h,8 h,1 d,3 d,7 d and 14 d after injury.The terminal deoxynucleotidal transferasemediated dUTP-biotin nick end labeling(TUNEL) method was used to detect the neural cell apoptosis and immunohistochemical method was used to observe the expressions of Bcl-2 and Bax.The results were analyzed by drawing diagram and statistic methods.RESULTS: TUNEL-positive cells were seen primarily at 4 h after injury,peaked at 8 h and then decreased,but few positive cells still exited at 14 d.The Bcl-2/Bax ratio kept minimal at 8 h and then gradually increased.The lower the Bcl-2/Bax ratio was,the more there were apoptotic cells.CONCLUSION: ① The apoptosis of neural cells and the expressions of bcl-2 and bax occur after the spinal cord injury in rats.② Cell apoptosis is related to the Bcl-2/Bax ratio after acute spinal cord injury in rats.The apoptotic cells are the most when the ratio is the lowest.
【CateGory Index】: R651.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 2 Hits
1 SUN Guo-Zhu~1, ZHANG Qing-Jun~2, ZHANG Geng-Shen~1, LI Jian-Feng~1~1Department of Neurosurgery, Second Hospital, Hebei Medical University, Shijiazhuang 050000, China, ~2Department of Neurosurgery, People's Hospital, Peking University, Beijing 100044, China;Effect of NF-κB,Bcl-2 and Bax on neuronal apoptosis in experimental presyrinx state[J];Journal of the Fourth Military Medical University;2006-05
2 SI Dao-Wen1,ZHOU Hong-Xia1,ZHANG Yu-Xin1,CHENG Ai-Guo2,ZHANG Zuo-Feng11Department of Anatomy,School of Basic Medicine,2Department of Orthopaedics,Affiliated Hospital,North China Coal Medical College,Tangshan 063000,China;Neuroprotective effect of progesterone on neurons after traumatic brain injury in rats and its mechanism[J];Journal of the Fourth Military Medical University;2007-17
【Citations】
Chinese Journal Full-text Database 2 Hits
1 CHEN Yi Jun, ZHANG Xiang, FEI Zhou, GU Jian Wen, LIU Wei Ping Institute of Neurosurgery of Chinese PLA, Xijing Hospital, Fourth Military Medical University, Xi'an 710033, China;Apoptosis in human brain gliomas with different grades of malignancy[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;2000-09
2 WANG Xian Hui 1, LIANG Mi Fang 2, LI De xin 2, Xu Jing 1, MI Li 1, CHEN Zhi Nan 1 1Cell Engineering Research Centre, Fourth Military Medical University, Xian 710033, China, 2Institute of Virology, Chinese Academy of Preventive Medicine;Expression of murine bcl-X_L gene in CHO cells[J];Journal of The Fourth Military Medical University;2003-24
【Co-citations】
Chinese Journal Full-text Database 4 Hits
1 ZHU Li-Hua~1, LI Shu-Ying~1, ZHOU Hong-Xia~2, XI Jin-Kun~3, ZHANG Guang-Ling~1, ZHOU Tian-Ji~4 ~1Department of Pathobiology, Faculty of Bioscience, ~2Department of Anatomy, Faculty of Basic Medicine, ~3Central Laboratory, North China Coal Medical College, Tangshan 063000, China, ~4Department of Pathobiology, School of Medical Technology, Jiangsu University, Zhenjiang 212000, China;Effect of bcl-2 gene transfection on the proliferation of human gastric epithelial cell GES-1[J];Journal of the Fourth Military Medical University;2006-21
2 HUI Wenzhong(Department of Nerosungery,Huaihe hospitol of Henan University,Kaifeng 475002);Expression of Alpha B-crystallin gene in human glioma[J];Medical Journal of Communications;2007-01
3 LU Jian,MA Chao,JIANG Lin(Lanzhou Institute of Biological Products,Lanzhou 730046,China);Establishment of CHO Cell Strain for Overexpression of bcl-2 Gene[J];Chinese Journal of Biologicals;2009-02
4 WANG Zhan-feng~1,LUO Yi-nan~1,HONG Xin-yu~1,JU Yan~1, SUN Yu-qin~2,HAN Hai-ling~2 (1.First Hospital of Jilin University,Changchun 130021,China; 2.Affiliated Hospital of Beihua University,Jilin 132011,China);Study on Anti-tumor Mechanism of Crude Extractive Ginseng Saponin Rh_2[J];Journal of Beihua University(Natural Science);2005-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 YI Cheng-la,CHEN An min,XU Wei guo,et al. (Department of Orthopedics,Tongji hospitol,Tongji Medical College,Huazhong University of Science and Techology,Wuhan 430030,China);Study of correlation between Caspase-3 expression and neural cell apoptosis after spinal cord injury in rats[J];Journal of Traumatic Surgery;2004-03
2 JIANG Dian-ming(Department of Orthopaedics,First Affiliated Hospital of Chongqing University of Medical Sciences,Chongqing 400016,China);Pharmacological interventions for acute spinal cord injury[J];Journal of Traumatic Surgery;2005-06
3 WANG Xian Hui 1, LIANG Mi Fang 2, LI De xin 2, Xu Jing 1, MI Li 1, CHEN Zhi Nan 1 1Cell Engineering Research Centre, Fourth Military Medical University, Xian 710033, China, 2Institute of Virology, Chinese Academy of Preventive Medicine;Expression of murine bcl-X_L gene in CHO cells[J];Journal of The Fourth Military Medical University;2003-24
4 LI Wen Lian, WANG Ling, ZHANG Hong Ai Department of Paediatrics, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China;Influence of Ca~(2+) on the phosphorylation of CREB, and the relationship between P-CREB and apoptotic neurons in neonatal rats after hypoxic-ischemia[J];Journal of The Fourth Military Medical University;2004-03
5 Liu Wenge,Wang Feng,Lin Jiajun,et al.Department of Orthopaedics, the Affiliated Union Hospital of Fujian Medical University,Fuzhou,350001,China;The expression and role of apoptosis in spinal cord injury[J];Fujian Meidical Journal;2002-06
6 CHEN Qian, ZENG Yuanshan+{*}, ZHANG Wei, CHEN Suijun=(Division of Neurosciences,Department of Histology and Embryology,Zhongshan Medical College,Sun YatSen University,Guangzhou 510080,China) ;;EFFECTS OF SCHWANN CELLS AND LNNA OF THE NEURONAL SURVIVAL AND AXONAL REGENERATION IN CLARKE'S NUCLEUS AFTER RAT SPINAL CORD INJURY[J];Acta Anatomica Sinica;2004-06
7 FU Qiang,HOU Tie sheng,LI Ming,et al. (Department of Othopaedics,Changhai Hospital,the Second military Medical University,Shanghai,200433,China);Apoptosis of neural cells after acute spinal cord injury in rats[J];The Journal of Cervicodynia and Lumbodynia;2001-03
8 WANG Ting-ting, WU Ji-hong, ZHONG Yu-chao, HU Guang-di, LIANG Jia, LV Hai-bo, WANG Jing (School of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, Beijing 100029);Influence of different electroacupuncture time on SOD and NO contents in rats with acute spinal cord injury[J];Journal of Beijing University of Traditional Chinese Medicine(Clinical Medicine);2008-06
9 CHEN Lei1,XU Hai-feng1,LI Shu-guang2(1.Department of Oral and Maxillofacial Surgery,Guangdong Medical College,Zhanjiang 524023; 2.Department of Oral and Maxillofacial Surgery,Foshan Second People's Hospital, Foshan 528000,Guangdong Province,China);Advances of Studies on Glial Cell Line-derived Neurotropic Factor and Facial Nerve Damage and Regeneration[J];Journal of Oral and Maxillofacial Surgery;2006-04
10 XIN Haili,REN Haoyang,WEI Yuning (Second bureau of Drugs ,general hospital of Chinese PLA,Beijing 100853);Progress in Drug Therapy for Spinal Cord Injury[J];Science Technology and Engineer;2005-16
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 SUN Guozhu1,LI Zhaohui1,ZHANG Qingjun2,ZHAO Zongmao1,ZHANG Gengshen11Department of Neurosurgery,The 2 nd Hospital of Hebei Medical University,Shijiazhuang 050000;2Department of Neurosurgery,People's Hospital of Peking University,Beijing 100044,China;Effect of protein kinase C on expression of NF-κB and IκB in experimental presyrinx state[J];Chinese Journal of Neurosurgical Disease Research;2008-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved