Effect of NF-κB,Bcl-2 and Bax on neuronal apoptosis in experimental presyrinx state

SUN Guo-Zhu~1, ZHANG Qing-Jun~2, ZHANG Geng-Shen~1, LI Jian-Feng~1~1Department of Neurosurgery, Second Hospital, Hebei Medical University, Shijiazhuang 050000, China, ~2Department of Neurosurgery, People's Hospital, Peking University, Beijing 100044, China  

AIM: To investigate neuronal apoptosis in experimental presyrinx state and the regulatory role and mechanism of NF-κB, Bcl-2 and Bax in rabbits. METHODS: The experimental syringomyelia models of rabbits were established by intra- cisternal injection of Kaolin. Neuronal apoptosis and the expressions of NF-κB, IκB, Bcl-2 and Bax were measured with TUNEL and immunohistochemistry in 1, 3, 7, 14 and 21 d after operation respectively . RESULTS: Compared with that in control group, the NF-κB expression in Kaolin group increased from the 1st day after operation, reached its peak at 3rd day, remained there till 7th day, and returned to a normal level at 14th day approximately. The IκB expression showed a negative correlation with NF-κB at the same time points. The Bcl-2 and Bax expressions increased from the 1st day, reached its peak at 7th day, remained there till 14th day, and then began to drop. CONCLUSION: In presyrinx state of experimental syringomyelia, the increasing expression of NF-κB mainly upregulates its target gene Bax, thus inducing neuronal apoptosis to involve in nervous function injury.

【Fund】： 河北省科技厅基金(002761100)
【CateGory Index】： R744

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