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Effect of cholecystokinin octapeptide on LPS-induced SOD mRNA expression and increase of NF-κB activity in rat vascular smooth muscle cells induced by LPS

MA Li-Qin, GU Zhen-Yong, DONG Guo-Kai, LI Na, GAO Feng, LIU Xia, MA Chun-Ling, CONG Bin, LING Yi-Ling Department of Forensic Medicine and Pathophysiology, Hebei Medical University, Shijiazhuang 050017, China  
AIM: To investigate the effect of cholecystokinin octapeptide (CCK-8) on SOD mRNA expression in rat thoracic aortic smooth muscle cells (TASMCs) induced by lipopolysaccharides (LPS) in vitro, and its mechanisms of signal transduction. METHODS: The TASMCs were isolated and cultured from rats and stimulated with LPS in the presence or absence of CCK-8(10 -10-10 -6 mol/L) or vehicle alone or together. The expressions of Mn SOD and Cu-Zn SOD mRNA were assayed by RT-PCR; the IкBa protein level was detected by Western blot. The expression and localization of P65 protein, a subunit of NF-κB, was assayed by immuocytochemistry. RESULTS: In rat TASMCs there were the basic expressions of Mn SOD and Cu-Zn SOD mRNA. As compared with vehicle action, LPS induced the upregulation of Mn SOD and Cu-Zn SOD mRNA, enhanced P65 protein expression in cytoblast (P0.05) but reduced IκBa protein expression in cytoplasma (P0.05). CCK-8 in a dose-dependent manner (P0.05) obviously inhibited the above-mentioned effects by LPS stimulation (P0.05). CCK-8 alone led to reduction in basic expressions of Mn SOD and Cu-Zn SOD mRNA (P0.05) but no change of P65 protein and IκBa protein expressions in TASMCs. CONCLUSION: CCK-8 inhibits LPS- induced Mn SOD and Cu-Zn SOD mRAN expressions by regulating NF-κB activity in rat TASMCs.
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