Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of the Fourth Military Medical University》 2007-03
Add to Favorite Get Latest Update

High cell-density fermentation of engineered E.coli and antitumor immunity of recombinant human MAGE3/HSP70 in vivo

HU Pei-Zhen1,LU Fan2,WANG Xiao-Gong2,LI Xia1,SI Shao-Yan1,HUANG Yang1,MA Bin1,ZHANG Xiu-Min1,SUI Yan-Fang11Department of Pathology, Xijing Hospital, 2Department of MolecularBiology and Biochemistry, School of Basic Medicine, Fourth Military Medical University,Xi’an 710033, China  
AIM: To realize high cell-density fermentation of MAGE3/HSP70 engineered E.coli,and to evaluate the antitumor immunity of MAGE3/HSP70 fusion protein in vivo. METHODS: High cell-density fermentation of MAGE3/HSP70 enginered E. coliwas carried out with constant soluble oxygen and continuous cultivation in self-control fermentor. The mouse was immunized by purified MAGE3/HSP70 fusion protein in E.coli, and the antitumor immunity was detected by ELISPOT and LDH release assay. RESULTS: High cell-density fermentation of MAGE3/HSP70 engineered E.coli was successful, and the expression of MAGE3/HSP70 in fermented bacterial was the same as the flask culture. The results of ELISPOT and LDH release assay showed that MAGE3/HSP70 generated tumor specific cytotoxic T lymphocyte (CTL) to damage tumor cel1. CONCLUSION: High cell- densityfermentation of MAGE3/HSP70 engineered E.coli was realizedand MAGE3/HSP70 fusion protein can generate specific CTL to kill tumor cel1.
【Fund】: 国家自然科学基金(30271464);; 全军医药卫生科研基金重点资助项目(012084)
【CateGory Index】: Q78
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 2 Hits
1 MA Jia Hai, SUI Yan Fang, YE Jing, HUANG Ya Yu, CHEN Guang Sheng, SONG Hong Ping, ZHANG Xiu Min Department of Pathology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710033, China;Cloning, prokaryotic expression and purification of tumor antigen gene MAGE-3[J];Journal of The Fourth Military Medical University;2004-06
2 MENG Jie-Ru, YAN Zhen, ZHAO Ning, ZHANG Ying-QiBiotechnology Centre, Department of Pharmaceutics, Fourth Military Medical University, Xi’an 710033, China;High cell density fermentation of engneering E. coli expressing tumor targeted interferon-α2a[J];Journal of The Fourth Military Medical University;2004-23
【Co-citations】
Chinese Journal Full-text Database 9 Hits
1 ZHANG Chun, CONG Yan-guang, HU Fu-quan, RAO Xian-cai (Department of Microbiology, College of Medicine, Third Military Medical University, Chongqing 400038, China);Reconstruction of engineered bacteria in preparing recombinant human peptide antibiotic β[J];Acta Academiae Medicinae Militaris Tertiae;2006-22
2 ZHANG Li-jun,ZHANG Hui-zhong,CHEN Guang-sheng,YE Jing,SHI Jian-guo(Department of Pathology,Fourth Military Medical University,Xi'an 710032,China);Construction of recombined adenovirus encoding human melanoma antigen-1 and its expression in hepatocellular carcinoma cells[J];Immunological Journal;2008-03
3 HU Ling~1,WANG Hongqi~2,CHENG Xiaoyan~2,CUI Najuan~1,HU Chenxia~2,LI Jianguo~2,YE Liping~3(1.Spleen-Stomach Institute,Guangzhou University of TCM,Guangzhou 510405,China;2.School of Basic Medicine,Guangzhou University of TCM,Guangzhou 510405,China;3.Nursing School,Guangzhou University of TCM,Guangzhou 510405,China);Effect of Herba Hedyotis Diffusae on Expression of Heat Shock Protein 70 in Hepatoma Cell Line H22[J];Jouranl of Guangzhou Uiversity of Traditional Chinese Medicine;2007-01
4 HU Pei-zhen1,LI Xia1,MA Bin1,ZHANG Xiu-min1,ZUO Jian-feng2,FU Jing-guo2,SI Shao-yan1,HUANG Yang1,SUI Yan-fang11Department of Pathology,Xijing Hospital,Fourth Military Medical University,Xi'an 710032;2Shaanxi Province Liposome Research Center,Xi'an 710075,China;Preparation and characterization of nanoliposome-encapsulated tumor specific antigen[J];Journal of Modern Oncology;2007-12
5 LI Xia1,HU Pei-zhen1,ZHANG Xiu-min1,HUANG Yang2,SI Shao-yan3,SUI Yan-fang1,CHEN Juan41Department of Pathology,the Fourth Military Medical University,Xi'an 710032,China;2Department of Emergency,Xijing Hospital,the Fourth Military Medical University,Xi'an 710032,China;3306th Hospital of PLA,Beijing 100101,China;4Depatment of Urology Surgery,the Second Affiliated Hospital,Xi'an Jiaotong University,Xi'an 710004,China;Study on protective effects against tumor relapse induced by nanoliposome - encapsulated tumor specific antigen MAGE3/HSP70[NL(MH)][J];Journal of Modern Oncology;2008-09
6 REN Bing-xiu~1 WANG Jing~1 ZHANG Lu~2 WANG Zhe~1 YANG Wei-dong~1 ZHANG Ying-qi~2 LI Guo-quan~1 HE Li-qing~2(1.Department of Nuclear Medicine,Xijing Hospital,the Forth Military Medical University of PLA,Xi'an 710032,China;2.Center of Biotechnology,the Forth Military Medical University of PLA,Xi'an 710032,China);Study of Indirect ~(99)Tc~m Labelling NGR-IFN-α2a[J];Journal of Isotopes;2008-04
7 GE Wei1, 2, SUN Yu-jing1, LI Yuan2, ZHANG Shan-hong2, ZHANG Xiu-min1, HUANG Yang1, HU Pei-zhen1, SUI Yan-fang1 1Department of Pathology, 2Department of Geriatrics, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China;Anti-tumor immune responses of nanoemulsion-encapsulated MHS vaccine[J];Chinese Journal of Cellular and Molecular Immunology;2008-05
8 WANG Min,ZHAO Wei,GAO Xiang-dong,ZHANG Lu-yong,YAO Wen-bingSchool of Life Science & Technology,China Pharmaceutical University,Nanjing 210009,China;Investigation of fermentation conditions of recombinant consensus interferon in E.coli[J];Journal of China Pharmaceutical University;2006-05
9 HU Pei-zhen1,ZUO Jian-feng2,FU Jing-guo2,LI Xia1,SI Shao-yan1,GE Wei1,ZHANG Xiu-min1,LI Zeng-shan11Department of Pathology,Xijing Hospital,Fourth Military Medical University,Xi'an 710032;2Shaanxi Province Liposome Research Center,Xi'an 710075,China;Preparation and antitumor immunity of long circulating Nano-Liposome encapsulated tumor specific antigen[J];Chinese Journal of Cellular and Molecular Immunology;2009-11
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 LIU Yong, XU Zhi Kai, YAN Yan, ZHANG Fang Lin, WANG Hai Tao, XUE Xiao Ping, WU Xing An Department of Microbiology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi'an 710033, China;Prokaryotic expression, purification and antigentic analysis of hantaan virus S gene 5’ terminal 311 bp fragment[J];Journal of The Fourth Military Medical University;2002-05
2 BIE Liang Feng, YU Wen Bin, CHENG Xiao Dong, SU Ming Quan, LIU Jia Yun Centre of Clinical Molecular Biology, Xijing Hospital, Fourth Military Medical University, Xian 710033, China;Cloning and expression of the human high mobility group-1 protein code gene[J];Journal of The Fourth Military Medical University;2003-03
3 JIA Zheng cai (Institute of Immunology, PLA, Third Military Medical University, Chongqing 400038, China)$$$$;Advances in research of tumor antigen MAGE-A3[J];Immunological Journal;2001-S1
4 MENG Jieru,YAN Zhen,ZHAO Ning et al (Biotechnology Center, The Fourth Military Medical University, Xi'an 710032);Stability of Biological Characteristics of Recombinant Bacterial Strain Ex-Tumor Targeted IFNα2a[J];Chinese Journal of Biologicals;2003-04
5 WU Wen, SUI Yan fang, YE Jing, LI Zeng shan, GUO Ai lin, YANG Xin wei, QU Ping, ZHANG Xiu min Department of Pathology, Fourth Military University, Xi'an 710032, Shaanxi Province, China;The cloning of tumor-associated gene MAGE in human hepatocellular cell line[J];Journal of Cellular and Molecular Immunology;2002-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved