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《Journal of the Fourth Military Medical University》 2007-07
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Effect of exogenous nucleotides on repair of damaged DNA in mouse thymocytes

WANG Lan-Fang1, LE Guo-Wei2, DAI Qiu-Ping11Department of Preventive Medicine, School of Basic Medicine, Tongji University, Shanghai 200092, China, 2Key Laboratory of Food Science and Safety of Ministry of Education, Southern Yangtze University, Wuxi 214036, China  
AIM: To investigate the effect of exogenous nucleotides on the repair of damaged DNA in mouse thymocytes in vitro. METHODS: Thymocytes from Kunming male mice were treated with N-methyl-N′-nitro-N-nitrosoguanidine (MNNG, 10 μmol/L) to induce DNA damage. DNA-damaged cells were cultured in basic or complete RPMI 1640 medium supplemented with different levels of nucleotides (0, 0.1, 1, 10 mmol/L). DNA damage and repair were analyzed at 2, 5 h with single-cell gel electrophoresis. RESULTS: Nucleotides had no significant effect on the percentage of comet cells at 2 h in basic medium (P0.05), but the percentage of comet cells decreased significantly in complete medium with nucleotides supplementation (P0.01). The nucleotides supplementation significantly decreased the percentage of comet cells at 5 h (P0.01). Comet tail length had no signi-ficant difference among groups at 2 h in basic or complete medium (P0.05). Nucleotides of 1, 10 mmol/L decreased comet tail length at 5 h in basic medium (P0.01) . The supplementation of 1, 10 mmol/L nucleotides decreased comet tail length at 5 h in complete medium (P0.01). The percentage of DNA-repaired cells was increased significantly with nucleotides supplementation between 2 and 5 h in basic (P0.05) or complete medium (P0.01). CONCLUSION: The supplementation of nucleotides can accelerate the repair of damaged DNA in mouse thymocytes in vitro. The effect of nucleotides is related with the supplemental levels of nucleotides.
【Fund】: 教育部高校骨干教师资助项目(G0202)
【CateGory Index】: R151
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【References】
Chinese Journal Full-text Database 2 Hits
1 QIAN Jia1,2 SHI Yong-hui1,2 MA Li-qin2 LE Guo-wei1,2(1.State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China;2.Institute of Food nutrition and Safety, Jiangnan University, Wuxi, Jiangsu 214122, China);Study on the antioxidant ability of 5′-adenosine(5′-AMP) monophosphate in vitro[J];Food & Machinery;2008-01
2 QIAN Jia1,2,SHI Yong-hui1,2,HE Qian2,LE Guo-wei1,2 (1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China;2Institute of Food nutrition and Safety, Wuxi, 214122, China);Study on theAntioxidant Ability of 5’-Cytidylic Acid In Vitro[J];Acta Nutrimenta Sinica;2008-03
【Citations】
Chinese Journal Full-text Database 3 Hits
1 CUI Da Xiang 1)** , ZENG Gui Ying 2) , WANG Feng 1) ,TIAN Fu Rong 2) , GUO Yan Hai 1) , XU Jun Rong 1) ,YAN Xiao Jun 1) , REN Dong Qing 2) ,SU Cheng Zhi 1) ( 1) Institute of Genetic Diagnosis of the F;Analysis of Genes Associated with Exogenous Nucleic Acids Improving the Repair of Intestinal Epithelium After γ Irradiation in Mice[J];Progress In Biochemistry and Biophysics;2001-03
2 WT5BZ]MA Ai guo, LIU Si chao, DU Wei, ZHANG Xiu zhen [WT6BX](Department of Human Nutrition, Qingdao Medical College, Qingdao 266021)[WT5FZ];EFFECT OF NUCLEOSIDES SUPLEMENTATION ON THE REPAIR OF DNA OXIDATIVE DAMAGE IN LYMPHOCYTES[J];ACTA NUTRIMENTA SINICA;2000-01
3 WANG Lan-fang , LE Guo-wei , SHI Yong-hui , WANG Tie-sheng1 (Institute of Feed Research, Southern Yangtze University, Wuxi 214036;1Jiangsu Institute of Atomic Medcine, Wuxi 214036, China);EFFECT OF DIETARY NUCLEOTIDES ON DNA DAMAGE OF THYMOCYTES IN MICE[J];Acta Nutrimenta Sinica;2004-04
【Co-citations】
Chinese Journal Full-text Database 7 Hits
1 WANG Qiu_zhen 1 ,MA Ai_guo 2,ˇ ,SUN Yong_ye 2 , XUE Mei_lan 2 ,LIANG Hui 2 (1.Department of Prevention,Medical College of Qingdao University,Qingdao266021,Shandong,China;2.Institute of Nutrition,Medical College of Qingdao University,Qingdao 266021,Shandong,China);Effect of High Dose Vitamin E and C on Antioxidative Activity and DNA Damage in Rats[J];Carcinogenesis,Teratogenesis & Mutagenesis;2006-04
2 SU Xiao-Ming1,ZENG Gui-Ying1,REN Dong-Qing1,ZHOU Xiao-Guang1,FANG Heng-Hu1,ZHAO Tao1,JIN Cheng1,XU Xiu-Li21Department of Irradiation Medicine,School of Preventive Medicine, 2Department of Clinical Laboratory,Xijing Hospital,Fourth Military Medical University,Xi'an 710033,China;Effects of intestinal RNA on MLN bacterial translocation and endotoxin in blood of mice after abdominal ~( 60)Coγ- irradiation[J];Journal of the Fourth Military Medical University;2006-09
3 WANG Lan-fang1,LE Guo-wei2,YU Ze-peng2,SHI Yong-hui2 (1. School of medicine, Tongji Univ., Shanghai 200092; 2. School of Food Science and Technology, Southern Yangtze Univ., Wuxi 214036, China);Effects of Dietary Nucleotides on Hepatic Gene Expression of Early-weaned Mice[J];Journal of Shanghai Jiaotong University(Agricultural Science);2006-04
4 QIAN Jia1,2 SHI Yong-hui1,2 MA Li-qin2 LE Guo-wei1,2(1.State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China;2.Institute of Food nutrition and Safety, Jiangnan University, Wuxi, Jiangsu 214122, China);Study on the antioxidant ability of 5′-adenosine(5′-AMP) monophosphate in vitro[J];Food & Machinery;2008-01
5 REN Dong-qing1,YANG Wen-qing2,SU Xiao-ming1,et al.(1. Department of Radiation Medicine,Faculty of Preventive Medicine,Fourth Military Medical University,Xi'an 710032,Shanxi,China;2. Department of Rehabilitation Medicine,Tangdu Hospital,Fourth Military Medical University,Xi'an 710038,Shanxi,China);Recovery Effects of Rat's Intestinal RNA on Post-abdominal-γ-irradiation Intestinal Mucosal Damages[J];Practical Preventive Medicine;2007-04
6 WANG Lan-fang1,DAI Qiu-ping1,ZHANG Jie-ping2(1.Dept.of Preventive Medicine,Medical College,Tongji University,Shanghai 200092,China;2.Dept.of Physiology,Medical College,Tongji University,Shanghai 200092,China);Effect of AMP on proliferation and apoptosis of IEC-6 cells[J];Journal of Tongji University(Medical Science);2009-05
7 SU Xiao-ming,ZENG Gui-ying,REN Dong-qing,et al.Department of Irradiation Medicine, Faculty of Military Preventive Medicine, Fourth Military Medical University,Xi'an 710032,China;Effect of intestinal RNA on intestinal mucosal barrier and bacterial translocation in mice after abdominal γ-irradiation[J];Chinese Journal of Radiological Medicine and Protection;2006-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Gao Ruomei, Zou Hong, Yuan Zhuobin (Department of Chemistry, Craduate School, University of Science and Technology of China, Beijing 100039);Study on the Auto-oxidation of Pyrogallol by Electrochemistry[J];Chinese Journal of Analytieal Chemistry;1997-03
2 Shi Yonghui et al. (Wuxi University of Light Industry,Wuxi City 214036);Role of Dietry Nucleotide in Animal Nutrition[J];ANIMAL SCIENCE ABROAD;2000-02
3 ZHANG Zun_zhen,HENG Zheng_chang,LI Hong, et al.Department of Environmental Health,School of Public Health,Sichuan University,Chengdu 610041;Study on the Susceptibility and Repair Ability of Oxidative Damage in Tissue Cells[J];Journal of Environment and Health;2002-01
4 Ma Janhua(Collegy of Biology Engineering , Jimei University, Xiamen 361021);Study on the Scavenger Activities of Methyl Salicylate against Hydroxyl Radical[J];Chemistry;2006-03
5 LIU Run zhi (Hunan Normal University, Changsha 410081, Hunan, China);The Effect of Deoxyribonucleic Acid(DNA) on the Activity of Glutathione Raductase(GSH-Px)[J];Acta Laser Biology Sinica;2002-04
6 Zhang Xuejun, Liu Lian, Si Chanping (Department of Microbiology, Anhui Medical University, Hufei 230032);1989年9月22日 EFFECT OF SCAVENGERS OF REACTIVE OXYGEN SPECIES AND Ca~(2+)-CHANNEL BLOCKER ON THE HUMAN LYMPHOCYTE CHEMILUMINESCENCE[J];Progress In Biochemistry and Biophysics;1990-06
7 PENG Chang Lian, CHEN Shao Wei, LIN Zhi Fang, LIN Gui Zhu(South China Institute of Botany,The Chinese Academy of Sciences, Guangzhou 510650,China).;Detection of Antioxidative Capacity in Plants by Scavenging Organic Free Radical DPPH.[J];Progress In Biochemistry and Biophysics;2000-06
8 ZHOU Yan-Hong, ZHANG Bi-Cheng, ZENG Zhao-Yang, ZHANG Wen-Ling, FAN Song-Qing, LI Xiao-Ling, XIONG Wei, CAO Li, LI Wei-Fang, LI Gui-Yuan (Cancer Research Institute, Xiangya School of Medical, Central South University, Changsha 410078, China);Screening The Differential Expression Genes of Nasopharyngeal Carcinoma Using GenMAPP[J];Progress In Biochemistry and Biophysics;2005-12
9 Jin Ming;Cai Yaxin;Li Jinrong;Zhao Hui(Beijing Heart Lung and Blood Vessel MedicalCenter;Beijing 100029;China).;1, 10-Phenanthroline- Fe ̄(2+) Oxidative Assay of Hydroxyl Radical Produced by H_2O_2/Fe ̄(2+)[J];PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS;1996-06
10 WEN Jing,HE Su-hua,YANG Yu-ying,TANG Xiu-hua(College of Arts and Sciences of Beijing Union University, Beijing 100083);The in Vitro Experimental Methods and Principles for the Eliminationof Free Radicals by Health Functional Foods[J];Food Science;2004-01
【Secondary References】
China Proceedings of conference Full-text Database 1 Hits
1 LE Guo-wei~1 SHI Yong-hui~2 (1.School of Food Science and Technology,Jiangnan University,Wuxi,214122,China; 2.Key Lab of Food Science and Technology,Jiangnan University,Wuxi,214122,China);Effect of Redox Status on Function of Animal Digestive System[A];[C];2008
【Secondary Citations】
Chinese Journal Full-text Database 7 Hits
1 Ma Aiguo 1,Andrew R.Collins 2,Susan J.Duthie 2 1Department of Human Nutrition,Qingdao Medical College,Qingdao 266021, 2The Rowett Research Institute,U.K.;THE DIFFERENCES OF DNA DAMAGE AND REPAIR BETWEEN SEVERAL TYPES OF CELLS[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;1997-03
2 Zeng Guiying;Han Shichen;Liu Jian;Liu Aiping;Zhou Yuankai Department of Radiation Medicine, Fourth Military Medical University, Xi'an;EFFECTS OF THE CELL-FREE FILTRATES OF THE CRYPT CELL SUSPENSION ON THE CRYPT REGENERATION IN THE IRRADIATED MICE[J];Journal of Radiation Research and Radiation Processing;1990-04
3 Zeng Guiying;Chen Binfu;Liu Aiping;Zhou Yuankai Fourth Military Medical University, Xian 710032;ISOLATION AND CHARACTERIZATION OF THE ACTIVE ENTITIES IN INTESTINE HOMOGENATE THOSE ENHANCING THE CRYPT SURVIVAL IN MICE AFTER γ-IRRADIATION[J];Journal of Radiation Research and Radiation Processing;1993-01
4 CUI Daxiang,ZENG Guiying,WANG Fen,TIAN Furong,YAN Xiaojun REN Dongqing ZHAO Tao, SU Chengzhi (Chinesc PLA Institute of Genetic Diagnosis, Fourth Military Medical University, Xi'an 710O32) (Department of Prevention Nucleic Weapon, Fourth Military Medical University Xi'an 710032);DIFFERENTIALLY EXPRESSED GENES IN REPAIRING IRRADIATION-INDUCED DAMAGE IN MOUSE INTESTINAL TRACT BY FOREIGN MOUSE SMALL INTESTINAL RNA[J];Journal of Radiation Research and Radiation Processing;2000-03
5 Zhou Yuankai; Liu Alping; Zeng Gulying(Department of Radiation Medicine, Fourth Military Medical University, Xi'an 710032);THE NUCLEIC ACID PRECURSORS CAN ENHANCE THE INTESTINAL CRYPT SURVIVAL IN MICE AFTER 980 cGy ABDOMINAL γ-IRRADIATION[J];Journal of Radiation Research and Radiation Processing;1994-02
6 Wang Jiao Zhou Yuankai (Department of Radiation Medicine,Fourth Military Medical University);EFFECTS OF CRYPT REPAIR FACTOR ON THE REPAIR AND REGENERATION OF CRYPTS OF LIEBERKUHN IN IRRADIATED MICE[J];Bulletin of the Academy of Military Medical Sciences;1990-03
7 Tang Xiaoli Xu Shibo Zhou Yonghong;The RadioProventive and DNARepairing Action of Exotic DNA from Carp Spermary[J];ACTA SCIENTIARUM NATURALIUM UNIVERSITATIS SUNYATSENI;1998-04
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