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《Journal of the Fourth Military Medical University》 2007-07
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Promoter hypermethylation and mRNA expression of TSLC1 gene in laryngeal squamous cell carcinoma

WANG Jun-Li1, ZHANG Hui-Zhong2, BAI Wan-Sheng1, LIU Li2, BIAN Ka1, CHENG Shi-Yin11Department of Otorhinolaryngology, 2Department of Clinical Laboratory, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China  
AIM: To explore the relationship between hyper-methylation of the promoter of TSLC1 gene and laryngeal squamous cell cancer. METHODS: Promoter hypermethylation and mRNA expression of TSLC1 gene were respectively detected by methylation-specific PCR and RT-PCR in normal laryngeal mucosa (n=4) and laryngeal squamous cell cancer (n=40). RESULTS: Among the 40 patients with laryngeal squamous cell cancer, hypermethylation of TSLC1 promoter was seen in 21 cases (52.5%). There was no significant difference in hypermethylation in relation to pathological grade and clinical stage and classification (P0.05), but a highly significant difference was observed between the patients with and without lymph node metastasis (N0 and N1) (P0.05). TSLC1 promoter hypermethylation was observed in human laryngeal squamous cell line Hep-2. TSLC1 mRNA was not expressed in all laryngeal squamous cell cancers with hypermethylation of TSLC1 promoter and Hep-2 cells, whereas it was expressed in normal laryngeal mucosa, polyp of vocal cord and laryngeal squamous cell cancers without hyper-methylation. CONCLUSION: Hypermethylation of TSLC1 promoter is associated with loss of its transcription in laryngeal squamous cell carcinoma.The high- frequency hypermethylation of TSLC1 promoter illustrates its potential clinical application as tumor marker for diagnosis and prognosis.
【CateGory Index】: R739.65
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