Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of the Fourth Military Medical University》 2007-07
Add to Favorite Get Latest Update

Promoter hypermethylation and mRNA expression of TSLC1 gene in laryngeal squamous cell carcinoma

WANG Jun-Li1, ZHANG Hui-Zhong2, BAI Wan-Sheng1, LIU Li2, BIAN Ka1, CHENG Shi-Yin11Department of Otorhinolaryngology, 2Department of Clinical Laboratory, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China  
AIM: To explore the relationship between hyper-methylation of the promoter of TSLC1 gene and laryngeal squamous cell cancer. METHODS: Promoter hypermethylation and mRNA expression of TSLC1 gene were respectively detected by methylation-specific PCR and RT-PCR in normal laryngeal mucosa (n=4) and laryngeal squamous cell cancer (n=40). RESULTS: Among the 40 patients with laryngeal squamous cell cancer, hypermethylation of TSLC1 promoter was seen in 21 cases (52.5%). There was no significant difference in hypermethylation in relation to pathological grade and clinical stage and classification (P0.05), but a highly significant difference was observed between the patients with and without lymph node metastasis (N0 and N1) (P0.05). TSLC1 promoter hypermethylation was observed in human laryngeal squamous cell line Hep-2. TSLC1 mRNA was not expressed in all laryngeal squamous cell cancers with hypermethylation of TSLC1 promoter and Hep-2 cells, whereas it was expressed in normal laryngeal mucosa, polyp of vocal cord and laryngeal squamous cell cancers without hyper-methylation. CONCLUSION: Hypermethylation of TSLC1 promoter is associated with loss of its transcription in laryngeal squamous cell carcinoma.The high- frequency hypermethylation of TSLC1 promoter illustrates its potential clinical application as tumor marker for diagnosis and prognosis.
【CateGory Index】: R739.65
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 3 Hits
1 WANG Bi-rong,LIANG Qi-lian Tumor Center of the Affiliated Hospital of Guangdong Medical College,Zhanjiang 524001,China;Relationship between a novel anti-oncogene TSLC1 and digestive apparatus tumor[J];Journal of Modern Oncology;2009-03
2 XIAO Zhong-di1,2,ZHANG Yu-cheng3,FANG Xue-dong1.(1.The Second hospital of Jilin University,Changchun 130041,China;2.General Surgery,Daqing Oil Field General Hospital;3.Central Laboratory,China-Japan Union Hospital,Jilin University);Influences of TSLC1 gene transfection of HepG2 hepatoma cell[J];Chinese Journal of Laboratory Diagnosis;2010-03
3 XIAO Zhongdi1,2,ZHANG Yucheng3,FANG Xuedong1(1.The Second Hospital of Jilin University,Changchun 130041,China;2.Department of General Surgery,Daqing Oil Field General Hospital,Daqing 163001,China;3.Central Laboratory,China-Japan Union Hospital,Jilin University,Changchun 130033,China);Construction of an eukaryotic expressing vector pReceiver-M29-TSLC1 and its expression in hepatoma HepG2 cells[J];Chinese Journal of Stereology and Image Analysis;2010-01
Chinese Journal Full-text Database 1 Hits
1 LI Ming, LOU Fang Ding, LU Xue Chun, JIN Hai Jie, YU Li Department of Hematology, General Hospital of PLA, Beijing 100853, China;The Study on Methylation of Gene IGSF4 Promoter in Acute Leukemia Cells[J];Journal of Experimental Hematology;2004-02
Chinese Journal Full-text Database 5 Hits
1 WANG Yongping, WU Cuihuan Department of Pathology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China;Advances in the Research of New Tumor Suppressor Gene tslc1[J];Journal of Medical Molecular Biology;2005-04
2 XU Shuo-qi(Experimental Center of Life Science,Nanjing Agricultural University,Nanjing 210095,China);Epigenetics in Tumor[J];Animal Husbandry and Feed Science;2010-02
3 WANG Hong-Bing,MIAO Hui,ZHANG Jing-Chuan,et al. Department of Oncology, Affiliated Hospital of Xuzhou Medical College,Xuzhou,221002;The Study of CpG Island Methylation of E-cadherin Gene Promoter in Lung Cancer[J];The Practical Journal of Cancer;2007-04
4 KANG Hui-Yuan1,WANG Chang1,2,DOU Li-Ping1,QING Yun1,HAN Xiao-Ping1,SUN Jing-Fen1,LIU Chun-Hui1,LU Xue-Chun3,LIU Yang1,3,YU Li11Department of Hematology,PLA General Hospital,Beijing 100853,China;2Department of Hematology and Oncology,The First Hospital of Jilin University,Changchun 130021,Jilin Province,China;3Department of Geriatric Hematology,PLA General Hospital,Beijing 100853,China;Methylation Status of ZO-1 Gene in Patients with Myelodysplastic Syndrome[J];Journal of Experimental Hematology;2008-01
5 DENG Xiaojing,GU Yan,LU Qin.Department of General Practice,Zhongda Hospital,Southeast University,Nanjing 210009, CHINA;Inhibitory effect of 5-AZA and gambogic acid on apoptosis of K562/A02 line[J];Jiangsu Medical Journal;2010-22
Chinese Journal Full-text Database 10 Hits
1 LI Na1,LI Fu-biao2,LIU Jie3,HAN Dan-dan1,CHEN Yan-ping4,MA Xing-yuan4,CHEN Xia1 (1.Department of Pharmacoclogy,School of Basic Medical Sciences,Jilin University,Changchun 130021,China;2.Department of Urinary Surgery,First Hospital,Jilin University,Changchun 130021,China;3.Experimental Center of Functional Sciences,School of Basic Medical Sciences,Jilin University,Changchun 130021,China;4.School of Chemistry,Jilin University,Changchun 130012,China);Protective effects of oxymatrine on injury induced by focal cerebral ischemia in rats[J];Journal of Jilin University(Medicine Edition);2008-03
2 LI Wen-Tao, BAI Hua-Long, ZHOU Chuang, SONG Yan, FENG Liu-Shun, ZHANG Shui-JunDepartment of General Surgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China;Expression of TSLC1 protein in human hepatocellular carcinoma and its clinical significance[J];Journal of the Fourth Military Medical University;2007-23
3 LI Dan,ZHANG Wei,LI Fumin,HUANG Jing,LUO RuoyuDept.of Obstetrics and Gynecology,Renmin Hospital of Wuhan University, Wuhan 430060,China;Effect of Matrine on Cervical Cancer HeLa Cells[J];Medical Journal of Wuhan University;2008-01
4 ZHAO Jun-fang1, XIE Wei-hong1, LI Xin-ming1, CHEN Wan-tao2, SUN Ming-lei1, FANG Zheng1,SUN Qiang1. (1. Dept. of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; 2. Dept. of Oral and Maxillofacial Surgery, The Ninth Affiliated Hospital of Shanghai Jiao Tong University, Shanghai 200011, China);Effect of Matrine on cell cycle and human telomerase reverse transcriptase of human salivary adenoid cystic carcinoma[J];West China Journal of Stomatology;2010-03
5 BAI Weiliang1 JI Wenyue1 REN Zhong2(1Department of Otolaryngology,Shengjing Hospital,China Medical University,Shenyang,110004,China;2Department of Otolaryngology,the First Affiliated Hospital,China Medical University);Relationship between the local invasion and the lymph node metastasis of the supraglottic carcinoma[J];Journal of Clinical Otorhinolaryngology Head and Neck Surgery;2008-04
6 CAO Ting-jia,YI Ji-lin,LU Qi-ping.(Department of General Surgery,Wuhan General Hospital,Guangzhou Military Area,Wuhan 430070,China);Cholangiocarcinoma associated genes differentially expressed by cDNA microarray[J];Journal of Clinical Surgery;2005-03
7 XIONG Jiang-tuan,LI Nan,JIANG Yi-deng (Dept.of Pathophysiology,Ningxia Med.Coll,Yinchuan 750004);An Experiments Study on Antilipdic and Anti-hyperoxidizative Effects of Matrine[J];Journal of Ningxia Medical College;2007-04
8 Wei Ke Si~1, Jing Pan~2, Hua Lu~2 and Zhao Quan Li~2 1 Department of Clinical Biochemistry, Third Military Medical University, Chongqing 400038 China 2 Department of Laboratory Medical Science, Third Military Medical University, Chongqing 400038 China;Study on matrine inhibiting proliferation of HepG2 cell and the relation between its dosage and inhibiting style[J];World Chinese Journal of Digestology;2001-02
9 Zhi-Qing Liu, Qiu Zhao, De-Min Li, Hua Qin, Yu Wang, Yu-Sheng Liao, Cui-Fang Zhang, Xiao-Yu Ke, Department of Gastroenterology, Tongji Hospital of Tongji Medical College, Huazhong Science and Technology University, Wuhan 430030, Hubei Province, China;Expression and clinical significance of TSLC1 and DAL-1/4.1B in pancreatic cancer[J];World Chinese Journal of Digestology;2008-31
10 LI Ming, LOU Fang Ding, LU Xue Chun, JIN Hai Jie, YU Li Department of Hematology, General Hospital of PLA, Beijing 100853, China;The Study on Methylation of Gene IGSF4 Promoter in Acute Leukemia Cells[J];Journal of Experimental Hematology;2004-02
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 QIAO Wei,JU Jian Department of Cadres Treatment Digestion Physicians,the Second Affiliated Hospital of Kunming Medical University, Kunming 650101,China;Relationship between DNA methylation and gastric cancer[J];Chinese Journal of Gastroenterology and Hepatology;2010-11
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved