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《Journal of the Fourth Military Medical University》 2007-18
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Effects of mechanical strain on expression of ERK1/2 in osteoblastic cells in vitro

CAO Yang1, ZHENG Yi2, CHEN Yang-Xi3, LUO Song-Jiao31Department of Orthodontics,Guanghua College of Stomatology, Sun Yat-sen University, Guangzhou 510055, China, 2Smile Research Center of Stomatology, Zhuhai 519000, China, 3Department of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu 610041, China  
AIM: To study the effect of mechanical strain on the expression of ERK1/2 in osteoblast-like cells in vitro. METHODS: Osteoblast-like cells were cultured and then subjected to mechanical strain by self-made four-point bending system at a 0.5 Hz frequency for 5, 15, 30 min, 1, 3, 6 h, respectively. In each time-phase, the cells were loaded with tension and compression stress at 4000 μ strain respectively. The phosphorylation levels of ERK1/2 were measured by Western Blot. Changes in the experiments were expressed as ratio to the controls. RESULTS: The self-made four-point bending system supplied an accurate and effective cyclic uniaxial strain to the cultured adhesion cells in vitro. 4000 μ strain tension stimulus and compress stimulus induced the expression of ERK in a few minutes and the effect of tension strain was more significant (P0.05). It may be due to the distinct mechanotransductive mechanism of the different directional mechanical strain. CONCLUSION: Mechanical strain plays an important role in the activation of signaling pathway of MAPK/EPK in osteoblasts . The effect of tension strain is more significant than compress strain. Mechanical strain is involved in the activation of extracellular signal regulated kinase.
【Fund】: 国家自然科学基金(39800145);; 广东省自然科学基金博士启动项目(5300622)
【CateGory Index】: R318.01
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【References】
Chinese Journal Full-text Database 2 Hits
1 JIANG Xue-mei1, YANG Chun1, YUAN Lin1, DIAO Jian-xin2, ZHANG Xue-quan1, HUANG Yong2, DAI Jing-xing1, QIU Xiao-zhong1, YU Lei1 1School of Basic Medical Sciences, 2School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China;Expression of extracellular signal-regulated protein kinases in the subcutaneous fascia of rats and their changes after acupuncture[J];Journal of Southern Medical University;2009-04
2 CAO Yang*, ZHENG Yi, LUO Song-jiao. * Guanghua School of Stomatology, Inshtitute of Stomatological Research, Sun Yat-sen University, Guangzhou 510055, China;The regulation of tyrosine protein kinase blocking agent and cytochalasin D to ERK1/2 under the mechanical force[J];Chinese Journal of Stomatological Research(Electronic Version);2008-04
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LIU Qing-meiCapital Institute of Physical Education,Beijing 100088,China.;Effects of Sports on p38MAPK[J];Journal of Capital Institute of Physical Education;2007-04
2 YUAN Lin+ 1 , YAO Da-wei+2, TANG Lei+1, HUANG Wen-hua+1, JIAO Pei-feng+1, LU Yun-tao+1, DAI Jing-xing+1, ZHANG Hui+1, HE Zhen-quan+3, ZHONG Shi-zhen+1(1Department of Anatomy,the First Military Medical University, Guangzhou 510515,China; 2Department of Anatomy, Hong Kong Chineses University, Hong Kong China; 3Guangzhou Chinese Medical University,Guangzhou 510405,China);A STUDY ON MORPHOLOGICAL BASIS OF CHINESE ACUPUNCTURE AND MOXIBUSTION FROM DIGITAL HUMAN BODY[J];Acta Anatomica Sinica;2004-04
3 Zhang Zhiying Dang Ruishan~△Zhang Chuansen Li Liang Ren Congli Chen Eryu (Department of Anatomy,Second Military Medical University,Shanghai 200433,China);Differential gene expressions of rat extracellular matrix before and after acupuncture in Housanli[J];Chinese Journal of Anatomy;2007-02
4 Wang Chunlci Yuan Lin Wang Jun Jiao Peifeng (Department of Anatomy,South Medical University,Guangzhou 510515,China);Contrast study on the line course of fascia meridians made by three dimensional reconstruction and classical meridians in human body[J];Chinese Journal of Anatomy;2007-03
5 DANG Ping, SHI Sheng-gen , SONG Ying-liang (Stomatologyical Collage, Fouth Military Medical University, Xian710032, China);Activation of p38 mapk phosphorylation in human periodontal ligment cells induced by mechanical stress[J];Chinese Journal of Prosthodontics;2003-02
6 YUAN Lin1, WANG Jun1, WANG Chun-lei1, SHEN Bao-lin1, DAI Jing-xing1, HUANG Yong21. Teaching and Research Section of Anthropotomy, Southern Medical University, Guangzhou 510515,China2. Teaching and Research Section of Acupuncture and Moxibustion, School of Chinese Medicine,Southern Medical University, Guangzhou 510515, China;Fasciology: A New Theory on the Human Self-supervision and Control System[J];Science & Technology Review;2006-06
7 CHEN Zai-hong,YAN De-quan,YIN Mei-nv.(Center of Stomatology,Taizhou Hospital,Linhai 317000,Zhejiang Province,China);Research on expression of pERK in human periodontal ligament cells under orthodontic mechanical force[J];Stomatology;2008-08
8 GONG Xiao-Wei, JIANG Yong ** (Department of Pathophysiology and Key Laboratory for Shock and Microcirculation of PLA, The First Military Medical University, Guangzhou 510515, China);Mechanisms of The Subcellular Localization and Stimuli-induced Translocation of MAP Kinases[J];Progress In Biochemistry and Biophysics;2003-04
9 CHEN Er yu, DANG Rui shan, CHEN Huan sheng, SHENG Xue yong, LIU Fang, JUN Jie Teaching and Research Section of Anatomy, the Second Military Medical University, 200433, China;The distribution of blood vessels and calcium at point Waiqiu[J];SHANGHAI JOURNAL OF ACUUNCTURE AND MOXIBUSTION;2000-01
10 ZHANG Zhi-ying~1,DANG Rui-shan~1,JIN Jian-hua~2,HUANG Xuan~3,REN Cong-li~1,LI Liang~1,CHEN Er-yu~1.1.Teaching and Research Section of Anatomy,Second Military Medical University, Shanghai 200433,China;2.Teaching and Research Section of Anatomy,Wenzhou Medical' College China;3.Year 2003 Enrolled Clinical Medical Student,Second Military Medical University,China;The Role of Point-regional Extracellular Matrix in Initial Acupuncture[J];Shanghai Journal of Acupuncture and Moxibustion;2006-08
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