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《Journal of the Fourth Military Medical University》 2007-19
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Effects of Allium fistulosum L.oil on in vitro culture of gastric cancer cell line

LUO Hai-Bin1, QI Wei21Department of Internal Medicine, Affiliated Hospital, Peking University, Beijing 100871, China, 2Department of Surgery, PLA 520 Hospital, Mianyang 621000, China  
AIM:To investigate the effects of Allium fistulosum L. oil (ALFO) on gastric cancer cell line and the possible signal molecular mechanism underlying apoptotic process induced by AFLO. METHODS: Human gastric cancer MGC80-3 cells were cultured in vitro and treated by AFLO. Then its growth inhibitory rate was counted and IC50 was measured. Apoptotic process was investigated with fluorescence microscope. Intracellular-free cal-cium content at different times was determined by flow cytometry. Intracellular cyclic adenosine monophosphate (cAMP) levels were determined by radioimmunoassay (RIA). RESULTS: With increasing the concentration of AFLO, cell growth inhibitory rates went up correspondingly 24 h after treatment with AFLO and reached 5.71%, 36.33%, 44.34%, 54.52% and 74.15% respectively. IC50≈65 μg/mL. Hoechst 33258 fluorescent staining showed that apoptosis appeared in some cells treated by AFLO (100 μg/mL). When MGC80-3 cells were incubated in the pre-sence of AFLO (100 μg/mL) from 1 to 12 h, the total apoptotic rates were 50.16%, 53.48%, 77.84%, 83.71% and 41.34%, respectively, and the early apoptotic rates were 50.15%, 33.25%, 11.69%, 4.65% and 0.84%, respectively. In MGC80-3 cells treated by AFLO (100 μg/mL) for 5 min to 12 h, free calcium contents were (71.00±6.08), (77.67±1.53), (79.67±1.53), (83.33±5.03), (76.00±6.06), (75.67±2.52),(72.33±4.24) and (65.00±2.00) nmol/L, respectively and cAMP concentrations were (5.76±0.36), (18.51±2.06), (20.40±1.65),(16.09±1.46),(12.16±0.78),(9.88±0.53),(9.47±0.78),(9.82±1.06) pmol/mg, respectively. CONCLUSION: AFLO can inhibit the growth of gastric cancer cell line MGC80-3 and induce the apoptosis through increasing intracellular Ca2+ and cAMP levels.
【CateGory Index】: R735.2
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