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Anti-tumor action of adenovirus mediated Survivin RNAi in human breast cancer-bearing nude mice

XIE Fu-Hua1,2,WANG Run-Xiu3,LI Li-Ping2,QIN Yan-Mei2,LIANG Nian-Ci2 1Department of Biochemistry and Molecular Biology,3Department of Nephronia,First Affiliated Hospital,Gannan Medical College,Ganzhou 341000,China,2Institute of Biochemistry and Molecular Biology,Guangdong Medical College,Zhanjiang 524023,China  
AIM:To research the growth of human breast cancer in nude mice after Survivin is knocked down by RNAi.METHODS:We constructed recombinant adenovirus with shRNA expression vector of survivin,and detected its titre.MCF-7 cells were injected into 20 nude mice to make the model of human breast cancer.The mice were treated with adenovirus,which carried the expression vector of survivin shRNA;the tumors were stripped and weighed to get the growth inhibition rate.Immunohistochemistry assay was done to detect the expression of Survivin.RESULTS:MCF-7 cells were seeded into nude mice and transplanted tumors were achieved in 90%(18/20)mice.Tumors of Ad-survivin group grew slower than the controls.All of the specimens were demonstrated as infiltrating ductal carcinoma.The growth inhibition rate of Ad-survivin group was 28.89%(P0.05).Optical density value of the survivin-expressed area was(15.63±1.54),being lower than that of control groups(30.45±6.72,30.25±6.58,P0.05).CONCLUSION:With the help of adenovirus,gene therapy targetting survivin,which is mediated by RNAi,may be a strategy of curing human breast cancer.
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