Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of the Fourth Military Medical University》 2008-19
Add to Favorite Get Latest Update

Anti-tumor action of adenovirus mediated Survivin RNAi in human breast cancer-bearing nude mice

XIE Fu-Hua1,2,WANG Run-Xiu3,LI Li-Ping2,QIN Yan-Mei2,LIANG Nian-Ci2 1Department of Biochemistry and Molecular Biology,3Department of Nephronia,First Affiliated Hospital,Gannan Medical College,Ganzhou 341000,China,2Institute of Biochemistry and Molecular Biology,Guangdong Medical College,Zhanjiang 524023,China  
AIM:To research the growth of human breast cancer in nude mice after Survivin is knocked down by RNAi.METHODS:We constructed recombinant adenovirus with shRNA expression vector of survivin,and detected its titre.MCF-7 cells were injected into 20 nude mice to make the model of human breast cancer.The mice were treated with adenovirus,which carried the expression vector of survivin shRNA;the tumors were stripped and weighed to get the growth inhibition rate.Immunohistochemistry assay was done to detect the expression of Survivin.RESULTS:MCF-7 cells were seeded into nude mice and transplanted tumors were achieved in 90%(18/20)mice.Tumors of Ad-survivin group grew slower than the controls.All of the specimens were demonstrated as infiltrating ductal carcinoma.The growth inhibition rate of Ad-survivin group was 28.89%(P0.05).Optical density value of the survivin-expressed area was(15.63±1.54),being lower than that of control groups(30.45±6.72,30.25±6.58,P0.05).CONCLUSION:With the help of adenovirus,gene therapy targetting survivin,which is mediated by RNAi,may be a strategy of curing human breast cancer.
【CateGory Index】: R737.9
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 7 Hits
1 DAI Guan Rong 2, CUI Long 1, GAO Jun 3, WANG Yuan He 2 (1.Department of General Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; 2. Department of General Surgery,Changzheng Hospital;3.Institute of Navy Medic;Preparation of an adenovirus expressing cytosine deaminase and observation on its tumor killing effect in vitro[J];Academic Journal of Second Military Medical University;2001-01
2 MA Yin bin, WU Cheng yi, CHEN Xin (Department of General Surgery, First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400016, China);Inhibitory effect of arsenic trioxide on survivin expression in heterologous graft model of human breast infiltrating duct carcinoma in nude mice[J];Acta Academiae Medicinae Militaris Tertiae;2004-16
3 MA Shuang-wei,WU Kai-nan,ZHONG Xiao-gang,KONG Ling-quan Department of General Surgery,First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,P.R.China;Study of the inhibitory effect of quercetin on transplantation tumor of breast carcinoma cell line MCF-7 in nude mice[J];Journal of Qilu Oncology;2004-03
4 ZHAO Bin-jie*, ZHAO Hong-yang, JI Han-sheng, et al. *Department of Neurosurgery, Union Hospital, Tongji Medical School, Huazhong University of Sciences and Technology, Wuhan Hubei 430022, China;Experimental Study of Treatment of Gliomas with Adenovirus Mediated Human p27Kip1 Gene in Rats[J];Chinese Journal of Clinical Neurosurgery;2006-03
5 SUN Cengyi *,HU Chaoquan,SUN Liansheng,et al. * Department of General Surgery,the Affiliated Hospital of Guiyang Medical College,Guiyang 550004,P. R. China;Effects of induction of a human OSM gene via recombinant adenovirus on growth of human pancreatic carcinoma cells and tumorigenecity in nude mice[J];Chinese Journal of Hepatobiliary Surgery;2004-04
6 ZHOU Jian-feng, CHEN Gang, LU Yun-ping, WANG Shi-xuan, MA Ding. Biomedical Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;Recombinant adenovirus vector-mediated herpes simplex virus thymidine kinase gene transfer followed by ganiciclovir administration effectively inhibits growth of human small-cell lung cancer in a murine xenotransplant model[J];Chinese Journal of Oncology;2004-02
7 Sheng-Quan Cheng, Department of Pediatrics, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China Wen-Liang Wang, Wei Yan, Qing-Long Li, Li Wang, Wen-Yong Wang, Department of Pathology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China;Knockdown of survivin gene expression by RNAi induces apoptosis in human hepatocellular carcinoma cell line SMMC-7721[J];世界胃肠病学杂志(英文版);2005-05
Chinese Journal Full-text Database 10 Hits
1 XIA Jun1,HU Bo1,CHEN Zhi-wen1,LIU Li-ming2(1.Department of Biochemistry and Molecular Biology,Bengbu Medical College,Bengbu 233030,Anhui,China;2.Suzhou Medical College,Suzhou University,Suzhou 215004,Jiangsu,China);Arsenic Trioxide Induced Apoptosis in MDA-MB-231 Cells and Its Influence on Expression of Survivin and Caspase-3 Proteins[J];Carcinogenesis,Teratogenesis & Mutagenesis;2008-02
2 DAI Guan Rong 1, GAO Jun 2, CUI Long 3, WANG Yuan He 1 (1. Department of General Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; 2. Department of General Surgery, Changzheng Hospital; 3. Naval Medical Research;Cloning of human B7-1 gene cDNA and preparation of adenovirus vector carrying B7-1[J];Academic Journal of Second Military Medical University;2001-04
3 YANG Pei-Li, LIU Yu-Huan, CAI Zai-Long , LOU Yong-Hua ,CUI Ying, YANG Zhi-Feng, MAO Ji-Fang( Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433,China ; Department of Obstetrics and Gynecology ,Changhai Hospital);Construction of recombinant adenovirus encoding PTEN cDNA[J];Academic Journal of Second Military Medical University;2004-05
4 XIE Fu-hua1a,2, WANG Run-xiu1b,LI Chan-wu3,LIANG Nian-ci2 (1a.Department of Biochemistry and Molecular Biology,1b.Department of Nephronia,First Affiliated Hospital, Gannan Medical University,Ganzhou Jiangxi 341000;2.Institute of Biochemistry and Molecular Biology, Guangdong Medical College,Zhanjiang Guangdong 524023;3.Medical Department,Hospital of Chinese Medicine of Xiajiang County,Xiajiang Jiangxi 331400);Research on Hunman Breast Cancer Model in Nude Mice[J];Journal of Gannan Medical University;2008-03
5 Yong-Mei Shen,Xiao-Chun Yang,Miao-Li Song,Chen-Hao Qin,Chen Yang and Yi-Hui Sun Radioimmunoassay Center & Clinical Laboratory,Department of MRI,and Department of General Surgery,Second Affiliated Hospital,Soochow University,Suzhou 215004,China;Radioimmunoassay Center,the Municipal Hospital of Suzhou,Suzhou 215002,China;Growth inhibition induced by short hairpin RNA to silence survivin gene in human pancreatic cancer cells[J];国际肝胆胰疾病杂志(英文版);2010-01
6 DI Hailing,KONG Li,LIU Junru,et al.The Third Hospital of Hebei Medical University,Shijiazhuang 050051;Effects of Survivin expression on cell proliferation,apoptosis and related clinicopathological features in hepatocellular carcinoma[J];Hebei Medical Journal;2008-09
7 LIU Jin-long,QIAN Qing,LIU Sun-liang,GUO Zhi-yuan,DU Qing,GUO Shi-ying1,LI Chao-jun1(Department of General Surgery,the First Affiliated Hospital of NJMU,Nanjing 210029;1School of Life Science,Nanjing Normal University,Nanjing 210097,China);The killing effect on pancreatic cancer cell in vitro by the radio-inducible TK suicide gene that controlled by Egr-1 promoter[J];Acta Universitatis Medicinalis Nanjing(Natural Science);2007-11
8 HE Jian-bin, ZHANG Ping, OU Li-wen, WANG Xiao-hua Department of Respiration, First Affiliated Hospital of Nanhua University, Hengyang 421001, P.R.China;Expressions of HIF-1α and P-Selectin induced by Suramin combination with Quercetin in lung adenocarcinoma in mice[J];Chinese Journal of Cancer Prevention and Treatment;2006-11
9 LI Run-qing1,SHAN Bao-en21.Center of Clinical Laboratory,Affiliated Beijing Youan Hospital of Capital Medical University,Beijing 100069,P.R.China2.Research Center,Affiliated Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,P.R.China;Correlation between anti-tumor activity of quercetin and the inhibitive effect on Wnt/β-catenin signaling pathway[J];Chinese Journal of Cancer Prevention and Treatment;2008-21
10 Pharmacological School, Jinan University XU Yan CAI Yu LIANG Shao-ling YANG Yan-xia;Research Advance of Anti-tumor Materia Medica Acting on Cellular Message Transmission Pathway[J];Shanghai Journal of Traditional Chinese Medicine;2005-08
【Secondary Citations】
Chinese Journal Full-text Database 6 Hits
1 Cui Long, Dai Guanrong, Meng Ronggui, Jin Guoxiang, Tu Yue, Wang Yuanhe (Department of General Surgery, Changhai Hospital, Second Military Medical University, Shanghai,200433);Killing effects of cytosine deaminase(CD) gene on human colorectal carcinoma in vitro and in vivo[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;1999-11
2 Bai Shaohuai*, Zhao Yangbing, Zhang Xuanbo, Li Hongjiang,Jing Jing, Xie Congyi, Chen Guojing. *Laboratory Animal Center, WCUMS, Chengdu 610041;Establishment of a Heterologous Graft Model for Human Breast Infiltrating Duct Carcinoma in Nude Mice[J];JOURNAL OF WEST CHINA UNIVERSITY OF MEDICAL SCIENCES;1999-02
3 Lu Yunping1 ,\ Wang Shian2 ,\ Zhou Jianfeng3 et al 1 Department of Gynecology and Obstetrics,Tongji Hospital, Tongji Medical University, Wuhan 430030 2Department of Cancer, Yangtze River Shipping General Hospital , Wuhan 4300103Department of Hema;Construction and Identification of AdenovirusHerpes Simplex VirusThymidine Kinase Genes Therapy Vectors[J];ACTA UNIVERSITATIS MEDICTNAE TANGJI;1999-03
4 Kong Lingquan Wu Kainan Lin Hui Department of Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing;Study of Inhibitory Effect of Quercetin on Neovascilarization of Experimental Mammary Carcinoma[J];Chinese Journal of Clinical Oncology;2001-04
5 CHEN Xin;WU Chen-yi. The First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400016, China;Effect of arsenic trioxide on the heterologous graft model for human breast infiltrating duct carcinoma in nude mice[J];Chinese Journal of Experimental Surgery;2003-08
6 ;基因治疗的研究现状与评价[J];Chinese Journal of Oncology;2002-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved