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《Journal of the Fourth Military Medical University》 2009-09
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Change of neuroglobin in rats during endotoxic shock

LIU Chao1,3, SUN Shan-Quan1, YU Jian-Bo3, XU Qiang2, CHEN Hai1, LI Hong-Yan1, GAO Bao-Bing11Laboratory of Neurobiology, 2Institute of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China, 3Department of Anesthesiology, Nankai Hospital of Tianjin, Tianjin 300100, China  
AIM:To study the changes and significance of neuroglobin(Ngb) expression in hippocampus, cerebrospinal fluid(CSF) and plasma in rats under endotoxic shock condition. METHODS: Seventy adult Sprague-Dawley(SD) rats were randomly divided into control group (0.5 mL normal saline was injected via vena caudalis)and experimental groups (0.5 mL endotoxin was injected via vena caudalis, 0.016 g/kg). The brain tissues were examined respectively at the time point of 3, 6, 12, 24, 48 and 72 h(n=10). The expression of Ngb over different periods was measured by enzyme-linked immunpsorbert assay(ELISA), Western-blot and immunohistochemical method(IHC). The brain water content(BWC) was measured by wet and dry weight method. RESULTS: ELISA showed that the Ngb concentrations in the plasma, CSF and hippocampus after endotoxin injection were significantly higher than those in control group(P0.01)and peaked at 48 h, which were 34.89, 22.01 and 14.01 mg/L respectively. Western Blot showed that the average molecular weight(Mr) of Ngb in each group was 17×103. The quantity of Ngb expression in the intervention groups was higher than that in the control group. The expression in 3 h group was the lowest and the expression in 48 h group was the highest. IHC showed that Ngb-immunoreactive product was located in the cytoplasm of the pyramidal neurons and was yellow-brown in color. The BWC in each group after endotoxin injection significantly increased(P0.01)and peaked at 48 h. The peak proportion of BWC was 0.8313. CONCLUSION: Our results indicate that there is a positive correlation between the up-regulation of Ngb expression and the severity of brain impairment. The up-regulation of Ngb expression might be one of the endogenous nuroprotective mechanisms under endotoxic shock condition.
【Fund】: 国家自然科学基金面上项目(30270437)
【CateGory Index】: R459.7
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