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《Journal of the Fourth Military Medical University》 2009-24
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Berberine inhibits lipopolysaccharide induced COX-2 expression via ERK signaling cascade pathways in human peripheral blood monocytes

WANG Qi-Zhang1,GUO Yi2,HAN Li-Min1,HUANG Shu-Liang2,ZHANG Yan-Bo2 1Department of Neurology,Shenzhen Shajing Affiliated Hospital,Guangzhou Medical University,Shenzhen 518104,China,2Department of Neurology,Second Clinical Medical College,Ji'nan University,Shenzhen 518020,China  
AIM:To investigate the inhibition of berberine(BBR) on lipopolysaccharide(LPS)induced COX-2 expression via ERK signaling cascade pathways in human peripheral blood monocytes(HPMC).METHODS:HPMC were isolated and cultured from whole blood and divided into 5 groups treated with null,LPS,LPS+BBR 25 μmol/L,LPS+BBR 50 μmol/L,LPS+BBR 100 μmol/L respectively for 30 min,6,12 and 24 h.Then monocytes were extracted for RT-PCR and westernblot analysis to examine COX-2 mRNA and protein activated expression of ERK signaling pathway.Meanwhile,ERK and inhibitor was incubated together with LPS stimulation to examine COX-2 mRNA and protein expression.RESULTS:At the four time points after treatment,the COX-2 mRNA and protein expression decreased at a low ebb at 12 h after BBR treatment(P0.05).In the group of BBR treatment at various time points(at 6,12,24 h after treatment) and three levels of dosage,ERK protein expression was inhibited significantly.At the concentration of 25 and 50 μmol/L,JNK protein expression was not inhibited significantly by BBR compared with LPS stimulation group(P0.05).Whereas with the treatment of BBR at the concentration of 100 μmol/L,JNK protein expression could be inhibited obviously compared with LPS stimulation group(P0.01).Human monocytes COX-2 mRNA and protein expression was inhibited statistically significantly while incubated with ERK and JNK pathway inhibitor,PD98059(P0.01).CONCLUSION:We conclude that BBR inhibits COX-2 mRNA and protein expression in a dose-dependent manner and ERK expression could be suppressed by BBR.BBR may inhibit COX-2 expression via ERK signaling pathway in HPMC.High concentration of BBR may inhibit COX-2 expression via JNK signaling pathway in HPMC.
【CateGory Index】: R285.5
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