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《JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY》 1997-03
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Determination of serum glutathione S transferase using common ultraviolet spectrophotometer

Wang Wenxue, Zhou Siyuan, Li Wenli Department of Toxicology, Fourth Military Medical University, Xi'an 710033  
Objective: The aim is to develop such a method as can determine activity of serum glutathione S transferase (GST) using common ultraviolet spectrophotometer instead of precious analyzer reported. Method: The optimal conditions in the GST catalyzed reaction and operation sequence were chosen to eliminate the interference from the spontaneous reaction and to increase the sensitivity and precision in the assay. Results: The average of activity of serum GST from fifty of health human was found to be 4.6±3.1; while the averages obtained from both normal and intoxicated with p acetaminophenol mice were 34.4±4.2 and 2157±524 ( P 0.01), respectively, which were essentially identical with the data obtained with exguesite analyzer reported in the literatures. Conclusion: The present method used to assay activity of serum GST will be convenient, economic, reliable and easier to promote the use.
【CateGory Index】: R331
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