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《JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY》 1997-03
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Analysis of Hantavirus genetic variations in suckling mouse with PCR SSCP method

Xia Guoqing 1, Liu Yanfang 1, Yang Shoujing 1, Yan Peisong 1, Feng Lei 2 1Department of Pathology, 2Central Laboratory, Fourth Military Medical University, Xi'an 710033  
Objective: To investigate whether Hantavirus underwent genetic alterations in infected suckling mice. Methods: Vero E6 cell line monolayers were infected with Hantavirus Chen strain for the virus propagation and 3 day old suckling mice were subsequently infected with the virus in culture medium supernatant. Virus RNAs were respectively extracted from infected cells and mice by acid guanidium thiocyanate phenol chloroform method. The RNAs were then reverse transcribed into cDNAs and the same parts of G2 encoding sequences of the virus were cloned individually from infected cells and mice by PCR. Single stranded DNA conformation polymorphisms of the cloned genes were analysed by the method of non denaturing polyacrylamide gel electrophoresis. Results: The single stranded DNA electrophoretic maps of the genes cloned from infected suckling mice and cells were apparently different. Electrophoretic rate of the single strand DNA of the gene cloned from infected mice was obviously quicker than the correspondent segment cloned from infected cells. Conclusion: Hantavirus could undergo direct genetic changes owing to different hosts.
【Fund】: 国家自然科学基金
【CateGory Index】: R365.51
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【Co-references】
Chinese Journal Full-text Database 7 Hits
1 Dong Chengliang Deng Changyan (The College of Animal Science and Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070);Progress of Examination Techniques for Gene Mutation[J];JOURNAL OF YELLOW CATTLE SCIENCE;1998-03
2 XIANGQian 1 ,YUShou-yi 2 ,WANGHong 2 ( 1 Departmentof MedicalTeachingandResearch,NanfangHospital,2 Departmentof Epidermiology,FirstMilitaryMed-icalUniversity,Guangzhou510515,China);gyrA gene mutations in quinolone-resistant Shigellae flexneri[J];Journal of First Military Medical University;2001-12
3 GAO Guofu *, YAO Shuxiang, SUN Xiudi, CHANG Runsheng, JIANG Yong, LUO Xuechang, JIN Ping, QIAO Youlin. * Cancer Institute, Chinese Academy of Medical Sciences, Beijing 100021, P.R.China;Establishment of cohort to study lung cancer in Yunnan tin miners[J];Chinese Journal of Lung Cancer;2002-02
4 LV WEN-Fa~1, ZHAO Jing~1, OUYANG Hong-sheng~2, LIANG Guan-sheng~2 1.Animal Science and Technoloy College of Jilin Agriculture University Changchun 130118, China;2.Faculty of Veterinary, PLA University Changchun 130062, China);Research Progress of Myostatin[J];Ecology of Domestic Animal;2004-02
5 Qi Yu Tang Ju Huang Liying (Paediatrics , First Teaching Hospital , Beijing Medical Unirersity);Study on Principles of DNA Single Strand Conformation Polymorphism[J];BASIC MEDICAL SCIENCES AND CLINICS;1997-02
6 Shan Xuesong, Zhang Yuan, Li Ning ( College of Animal Science and Technology ,China Agricultural Univer sity,Beijing 10094; National Laboratory for Agro-Biotechnology,China Agricultural University,Beijing 100094);Partial Cloning and Analysing of Weaver Gene in Xinjiang Brown Cattle[J];JOURNAL OF AGRICULTURAL BIOTECHNOLOGY;2000-03
7 Li Guohua; Zhang Yuan; Li Ning ( College of Animal Science and Technology, China Agricultural University; The Nationa1 Laboratories for Agrobiotechnology China Agricultural University, Beijing l00094);Analysis On the Partial Sequence of Bovine Lactoferrin Gene by PCR-SSCP[J];Journal of Agricultural Biotechnology;2001-02
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