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《Freshwater Fisheries》 2008-02
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A Preliminary Study of Foot and Mantle Tissues Culture from Ampullaria gigas

JIANG De-xun1,XU Pu2,SHEN Ai-guo3,QIN Yong-wei3,XU Jian-rong2,KONG Xiang-ting2,SHEN hui4,YANG Jia-xin1 (1.College of Life Sciences,Nanjing Normal University,Nanjing 210097;2.Department of Biology and Food Engineering,Changshu Institute of Technology,Changshu,Jjiangsu 215500;3.School of Basic Medical Science,Nantong University,Nantong,Jiangsu 226001;4.Jiangsu Institute of Marine Fisheries,Nantong,Jiangsu 226007)  
The cells of foot and mantle tissues derived from Ampullaria gigas Spix were cultured in vitro with the modified medium 199.At 6 h post-inoculation,cells began to migrate out from the tissue.At 3 d post-inoculation the outgrowth was formed around the tissues.Being cultured for 21~28 days,most of the bottom of culture flask was covered by the cells.The cells of foot and mantle tissues have been subcultured to the eighth and ninth generation,respectively.There were two types of cells in the preliminary cell culture of the foot and mantle tissues.One was small epithelium-like cells,which were rounded,elliptic or polygonal with diameter of 7~15 μm;the other was middle epithelium-like size and large cells,which was elliptic and polygonal with diameter of 15~30 μm.There were plenty of the small epithelium-like cells,Which proliferated faster and could form cell monolayer in the preliminary culture and subculture.The results of preliminary culture and subculture showed that the growth ability and adherence of mantle tissue cells was better than that of foot tissue ones.The result of Hoechst fluorescein stain revealed that the apoptosis index of mantle tissue cells was apparently less than that of foot tissue cells.The present study implied that the mantle tissue could have potential to be established as a continuous cell line.
【CateGory Index】: S917.4
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