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《Freshwater Fisheries》 2009-02
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A preliminary study on snout tissue culture from Pelteobagrus fulvidraco

KONG Xiang-ting1,SHEN Song-dong1,JIANG De-xun2,JIANG Hong-xia2,XU Jian-rong2,ZHANG Jun2,HAN Xiao-lei2,XU Pu2(1.Department of Cell Biology,Soochow University,Suzhou,Jiangsu 215123;2.Department of Biology and Food Engineering,Changshu Institute of Technology,Changshu,Jiangsu 215500)  
The cells of snout tissues derived from Pelteobagrus fulvidraco were studied in vitro with the medium 199.The snout cells have been subcultured to the eighth generation.The snout cells migrated from the tissues after cultured in vitro for 3~5 h.After that,cells could be done once every 4or 6 days.The cells were fiber-like cells in primary culture and subculture.When cultured under different temperature(15,27,37 ℃) and serum concentration(0,10%,20%),cells were most quickly multiplied under 27 ℃ and 10% serum concentration.Chromosome research demonstrated that the snout cells in vitro were normal diploid cells(2n=52).The results showed that the snout tissue of P.fulvidraco reproduced rapidly and cultivated cells could adapt broad temperature in vitro.Therefore,they had the potential ability to become the tissue source of establishing continuous cell line.
【CateGory Index】: S917.4
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