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《Freshwater Fisheries》 2009-04
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Construction of muscle cDNA library and analysis of some expressed sequence tag sequences of Siniperca chutasi

MENG Tao1,2,DING Feng3,CHU Wu-ying2,ZHOU Rui-xue1,2,BING Shi-yu1,HU Song-nian3,ZHANG Jian-she2(1.The College of Life Science,Guangxi Normal University,Guilin 541004;2.Department of Bioengineering and Environmental Science,Changsha Uiversity,Changsha 410003;3.Beijing Institute of Genomics,Chinese Academy of Sciences,Beijing 100029)  
The cDNA library of Siniperca chutasi′s muscle was constructed by non-normalizad cDNA library construction techniques.The library capacity was 2.3×105,the recombination frequency was 96.5%,and the average size of inserts was 1.2 kb.Of total 5456 sequencing reactions,5191 were successfully sequenced,in which 5063 were in high-quality,which was 97.5%.1625 non-repetitive sequences including 443 contigs and 1182 singlets were analyzed with PHRAP software.The 1625 unique sequences were functionally related to muscle structure and myogenesis as searched from NCBI databases.991 of 1625 unique sequences were found to have high similarity to other fish species and the rest of 634 sequences were unique in S.chutasi accounting for 39 percent of the whole.Further,740 functional known genes were categorized by Gene ontology(GO).
【Fund】: 国家自然科学基金面上项目(30771644);; 湖南省自然科学基金项目(09JJ6037 08JJ064)
【CateGory Index】: S917
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