Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Freshwater Fisheries》 2012-02
Add to Favorite Get Latest Update

The karyotype and lsozymes of hybrid turtle(Chinemys reevesii♀×Ocadia sinensis♂) and comparison with their parents

HE Gang1,2,HE Li1,FANG Chun-lin2,XU Ying-fang1,FEI Chun-ping3,DING Li-yun2(1.Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences, Ministry of Agriculture,Wuhan 430223,China; 2.Fisheries Research Institute of Jiangxi Province,Nanchang 330039,China; 3.Jiangxi Jinguiwang Business Co.Ltd,Shangrao 334400,Jiangxi,China)  
The karyotypes of hybrid(Chinemys reevesii♀×Ocadia sinensis♂) and their parents were studied from kidney cells by the method of PHA and colchicine injection,and air-dry technique.The results showed that their chromosome formula were 2 n=52,18 m +10 st +24 t,and arm number(NF) was 80.LDH,EST,POD and ADH of five tissues(muscle,heart,liver,spleen and kidney) of C.reevesii,O.sinensis and their hybrid were detected by using vertical polyacrylamide gel electrophoresis.The relationship and the genetic diversity were studied.In 4 isozymes(LDH,EST,POD,ADH),a total of 50 loci were detected,15 in C.reevesii,16 in O.sinensis and 19 in the hybrid.Ldh-3,Pod-2 and Pod-3 were polymorphic,with the mean proportion of polymorphic loci being 13.3%,12.5% and 15.8%.The observed heterozygosity were 0.0256,0.0417 and 0.1023,expected heterozygosity were 0.0416,0.0407 and 0.0746,and genetic deviation index were-0.3864,0.0237 and 0.3725,suggesting that the hybrid had a higher genetic diversity than their parents.Comparison showed that the hybrid was closer to the C.reevesii than to the other in POD.POD and LDH bands detected in liver,POD bands detected in heart could serve as the biochemical markers for distinguishing C.reevesii,O.sinensis and their hybrid.
【Fund】: 国家公益性行业(农业)科研专项(200903048;201003076);; 农业部长江中上游渔业资源环境重点野外科学观测试验站开放课题(YWTZ/1001)
【CateGory Index】: S966.5
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 HE Gang;HE Li;FANG Chun-lin;FEI Chun-ping;CHENG Bi-jun;Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences;Scientific Observing and Experimental Station of Fishery Resources and Environment in Poyang Lake,Ministry of Agriculture / Fisheries Research Institute of Jiangxi Province;Scientific Observing and Experimental Station of Fishery Resources and Environment in Poyang Lake,Ministry of Agriculture/ Fisheries Research Institute of Jiangxi Province;Jiangxi Jinguiwang Business Co.,Ltd.;Institute of Agricultural Quality Standards and Testing Technology Research,Hubei Academy of Agricultural Sciences;;Study on the Phylogenetic Relation of Hybrid Turtle(Chinemys reevesii♀×Ocadia sinensis♂) Based on Cyt b Gene[J];Hubei Agricultural Sciences;2013-17
【Citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Ming, \ NIE Liu wang, \ GUO Chao wen (Department of Biology, Anhui Normal University, 241000, Wuhu, Anhui, China);The Karyotype and NORs of Ocadia sinensis From China[J];JOURNAL OF ANHUI NORMAL UNIVERSITY(NATURAL SCIENCE);1999-01
2 HE Gang1,2,HE Li2,XU Ying-fang2,ZHANG Zheng2,LI Xiao-li2 (1.Fisheries Colleges,Huazhong Agricultural University,Wuhan 430070;2.Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Jingzhou,Hubei 434000);Study on Germplasm Characters of Triplophysa xiangxiensis[J];Freshwater Fisheries;2008-02
3 Huang Shengmin Qin Changgeng Pan Shuying (Kunming Institute of Zoology, Academia Sinica) Wang Xiuyong Li Xiaohui Tion Shukui (Yunnan Fisheries Research Institute);COMPARATIVE STUDIES ON THE ELECTROPHOREGRAM OF ESTERASE ISOZYME AND LACTATE DEHYDROGENASE OF CARASSIUS AUKATUS GIBELIO BLOCH AND CARASSIUS SP.[J];Zoological Research;1988-01
4 ZHU Xin ping 1,2 ,CHEN Yong le 2,ZHANG Jin 1,DU He jun 2,GUI Jian fan 1 (1.State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology, the Chinese Academy of Sciences,Wuhan 430072,China; 2.Pearl River Fisheries Research Institute,Chinese Academy Fishery of Sciences,Guangzhou 510380,China);Cellular DNA Contents of Asian Yellow Pond Turtles (Mauremys mutica Cantor)[J];Zoological Research;2004-02
5 Fang Ling,Hu Qiping (Department of Biology,Guangxi Medical University,Nanning 530021);THE STUDY ON THE KARYOTYPE OF TESTUDO IMPRESSA (GUENTHER)[J];JOURNAL OF GUANGXI MEDICAL UNIVERSITY;1999-06
6 YANG Tian-yan GAO Tian-xiang(Fishery College,Ocean University of China,Qingdao 266003);Isozyme analyses of Trichiurus haumela in the Yellow Sea and East China Sea[J];Marine Fisheries Research;2007-03
7 Hu Tingmao Liu Minqiu Wang Huailai Tian Xuejue(Department of Biology,Nei Menggu University.Nei Menggu Fisheries Research Institute,Hohhot,010031);Study on Electrophoresis of Isozymes of Cyprinus carpio L. Yellow River carp,Seattered Cyptinus carpio L.mirror and their Hybrid F_1[J];ACTA SCIENTIARUM NATURALIUM UNIVERSITATIS NEIMONGOL;1994-03
8 PENG Jing,CHEN Ye-zhi,LI Gui-sheng(Department of Biotechnology,Jinan University,Guangzhou 510632);Esterase Isozyme of Annamemys annamensis Before and After Hibernation[J];Sichuan Journal of Zoology;2008-01
9 Zhu Lanfei(Institute of Hydrobiology, Academia Sinica, Wuhan 430072);GRADIENT GEL ELECTROPHORESIS ON POLYACRYLAMIDE OF ISOZYMES AND PROTEINS OF FISHES[J];Acta Hydrobiologica Sinica;1992-02
10 PAN De-Bo1,CHEN Kun-Ci1,ZHU Xin-Ping1,2,ZHENG Guang-Ming1,LIU Yi-Hui1,CHEN Yong-Le1 and LI Kai-Bin1(1. Pearl River Fisheries Research Institute,Chinese Fishery Academy of Sciences,Guangzhou 510380,China; 2.Colledge of life Science,Guangzhou University,Guangzhou 510006,China);THE MORPHOLOGIC CHARACTERS OF HYBRID(MAUREMYS MUTICA ♀× CUORA TRIFASCIATA ♂) AND COMPARISON WITH THEIR PARENTS[J];Acta Hydrobiologica Sinica;2009-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Xiao-hong,ZHANG Hu-fang,WANG Qing(Department of Biology,Taiyuan Teachers College,Taiyuan 030031,Shanxi,China);Effect of paraquat on the expressions of peroxidase and esterase in goldfish serum[J];Carcinogenesis,Teratogenesis & Mutagenesis;2010-05
2 WEN Xiao-lei1, JI Zhi-xin1, WANG Chang-qing2, WU Yan-chao3, LI Chun-hua4, DONG Hong-ping4* 1. College of Life Science and Technology, Hebei Normal University of Science & Technology, Qinhuangdao 066600, China; 2. Library of Hebei Normal University of Science & Technology, Qinhuangdao 066600, China; 3. Guantao County Bureau of Agriculture, Guantao 057750, China; 4. Europe and America College, Hebei Normal University of Science & Technology, Qinhuangdao 066600, China;Comparison on Esterase Isozymes and Peroxidase Isozymes of Black Worm Tenebrio molitor Linnaeus at Different Developmental Stages[J];畜牧与饲料科学(英文版);2009-Z2
3 Xue Ming et al (Fisheries College,Zhanjiang Ocean University, Zhanjiang,Guangdong 524025);Biochemical Genetic Variation in Two Populations of Huaihe Carp[J];Journal of Anhui Agricultural Sciences;2003-06
4 ZHA Xue-qiang et al(College of Biotechnology and Food Engineering,Hefei University of Technology,Hefei,Anhui 230009);Protein and Isoenzyme Analysis of Dendrobium officinale from 8 Producing Areas[J];Journal of Anhui Agricultural Sciences;2007-27
5 JIN Xi-biao et al (Rice Research Institute, Sichuan Agriculture University, Wenjiang, Sichuan 611130);Analysis on the Isozymes Changes in the Differentiation Stage of Young Panicle of Isonuclear Alloplasmic Rice Plant[J];Journal of Anhui Agricultural Sciences;2008-08
6 HONG Da et al (College of Life Science,Henan Normal University,Xinxiang,Henan 453007);Analysis of the Sex-linked Isozyme Marker in Cephalanoplos segetum(Bunge) Kitam[J];Journal of Anhui Agricultural Sciences;2008-09
7 LI Peng et al(College of Life Science,Ludong University,Yantai,Shandong 264025);Observation on the Chromosomes in 2 Species of Bivalve[J];Journal of Anhui Agricultural Sciences;2008-12
8 MENG Yan et al(Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Jingzhou,Hubei 434000);Comparative Studies on the Isozymes of Channa asiatica and Channa argus[J];Journal of Anhui Agricultural Sciences;2009-27
9 WEN Xiao-lei et al(Hebei Normal University of Sicence & Technology,Qinhuangdao,Hebei 066000);Comparison on Esterase Isozyme and Peroxidase Isozyme of Black Worm Tenebrio molitor Linnaeus at Different Developmental Stages[J];Journal of Anhui Agricultural Sciences;2010-03
10 MA Yong-ping et al (College of Biological Science and Engineering,Northern University for Nationalities,Yinchuan,Ningxia 750021);Analysis of the Genetic Diversity of Chinese Wolfberry at the Levels of Various Isozyme[J];Journal of Anhui Agricultural Sciences;2010-08
China Proceedings of conference Full-text Database 9 Hits
1 Jiang Xiangmei Wen Qiang Ye Jinshan Xiao Fuming Jiang Mei (Jiangxi Provincial Bio-tech Key Lab for Plant,Nanchang 330032);Population Genetic Diversity Analysis by RAPD in Phoebe bournei[A];[C];2006
2 Zhang Jie Chen Liqiao Zhou Zhongliang Tang Sixian;Study on the Developmental Genetics of Isozymes in Chinese Mitten-handed Crab I.Discussion on Isozymes in Various Tissues of Chinese Mitten-handed Crab from Changjiang River[A];[C];1996
3 ZHOU Jun-yi,BI Yu-fen,ZHANG Feng-xian (Department of Grassland Science,Faculty of Animal Science and Technology, Yunnan Agricultural University,Kunming 650201,China);Isoenzyme Analysis on Hybrid Progeny of Alfalfa[A];[C];2009
4 LU Fu-Ping, LI Cui-Lan, DUAN Yi-Hao, GUO Ya-Ping, MA En-Bo Environment and Plant Protection Institute, CATAS, Danzhou 571737, China College of Life Science and Technology, Shanxi University, Tatyuan 030006, China College of Environmental Science and Resources, Shanxi University, Taiyuan 030006, China;Relationship between malathion acute toxicity and allozyme genotypes of an Oxya chinensis population from Jinyuan, Shanxi[A];[C];2005
5 DOU Li-Zheng SAI Dao-Jian MIAO Xiu-Lian HUANG Ji-Zhi SONG Zi-Yu YE Ning (Life Science College , Shandong Normal University , Jinan 250014 , China);The circulatory model in the heart and principal blood vessels of impressed tortoise (Manouria impressa )[A];[C];2005
6 Li Yue, Zhang Chunxiao (College of Forest Resources and Environment, Beijing Forestry University, Beijing 100083);THE ISOZYME VARIATIONS AMONG POPULATIONS WITHIN ONE IMPROVEMENT SYSTEM OF PINUS TABULAEFORMIS CARR.[A];[C];1998
7 1Zhang Chunxiao, 1Li Yue, 1Shen Xihuan, 2Chen Xuemei (1College of Forest Resources and Environment, Beijing Forestry University, Beijing 100083) (2Department of Biology, Beijing Forestry University, Beijing 100083);GENETIC DIVERSITY OF TEN NATURAL AND ARTIFICIAL POPULATIONS OF PINUS TABULAEFORMIS IN TWO ISOZYME SYSTEMS[A];[C];1998
8 Chen Shaoyu Zhao Wenshu (Yunnan Academy of Forestry, Kunming 650204);Genetic Diversity and Differentiation of Pinus kesiya Royle Gord. var. langbinaensis Natural Populations[A];[C];2000
9 LI Bin and GU Wanchun (Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091);Conservation Genetics of Pinus bungeanaⅡ:Evaluation and Conservation of Natural Population'Genetic Diversity[A];[C];2002
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 YU Hong-Wei① ZHU Dong-Fa① HAN Bao-Qin②(① Faculty of Life Science and Biotechnology,Ningbo University,Ningbo 315211; ② College of Marine Life Sciences,Ocean University of Qingdao, Qingdao 266003,China);The Isozymes in Different Tissues of Portunus triuberbuculatus[J];Chinese Journal of Zoology;2005-01
2 BAO Di1,LIANG Ai-jun1,DONG Ying1,WANG Song1,JIN Wan-kun2,DONG Shi1(1.College of Life Sciences,Tianjin Normal University,Tianjin Key Laboratory of Cyto-Genetical and Molecular Regulation,Tianjin 300387,China;2.National Tianjin Huanxin Aquatic Thoroughbred Hatchery, Tianjin 301500,China);The Isozyme Electrophoresis in Hybrid of Crucian Carp with Common Carp[J];Fisheries Science;2012-05
3 Wang Xiufen (Dept.of Agron., Hebei Agri. Univ., Baoding, China);A Good Dyeing Method for Superoxide Isoenzyme[J];Journal of Agricultural University of Hebei;1990-04
4 HE Gang1,2,HE Li2,XU Ying-fang2,ZHANG Zheng2,LI Xiao-li2 (1.Fisheries Colleges,Huazhong Agricultural University,Wuhan 430070;2.Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Jingzhou,Hubei 434000);Study on Germplasm Characters of Triplophysa xiangxiensis[J];Freshwater Fisheries;2008-02
5 ZHANG Hai-Qi~1,HUANG Li-Ying~2,HE Zhong-Yang~1,DING Shi-Hua~21. Zhejiang Fisheries Introduction and Breeding Center,Hangzhou 310012, China2. College of Aquacluture and Hydrology, Southwest Agricultural University,Chongqing 400716, China;PRELIMINARY STUDY ON MITOCHONDRIAL CYTOCHROME b DNA SEQUENCES AND PHYLOGENY OF PELODISCUS SINENSIS FROM TAIHU CULTURED POPULATION AND JAPAN-INTRODUCED POPULATION[J];Acta Zootaxonomica Sinica;2006-02
6 ZHANG Yong-Zheng1,ZHANG Hai-Qi2,HE Zhong-Yang2, XU Xiao-Lin2,LI Zhi-Qiang1,DU Jian-Ming2(1.Xiaoshan Science and Technical College of Zhejiang Ocean University,Hangzhou,311258;2.Zhejiang Fisheries Introduction and Breeding Center,Hangzhou,310012);SEQUENCE VARIATION AND POPULATION GENETIC STRUCTURE OF FIVE POPULATIONS IN PELODISCUS SINENSIS BASED ON CYTOCHROME b GENE[J];Oceanologia Et Limnologia Sinica;2008-03
7 CHEN Yin-er,WANG Guo-liang,JIN Shan,LI Zheng(Life Science and Technology Falcuty,Ningbo University,Ningbo 315211,China);Analysis of the myogen and isozymes of the Portunus trituberculatus with emusification disease[J];Marine Sciences;2008-05
8 DONG Xu-hui,LI Ming-yun(Faculty of Life Science and Biotechnology,Ningbo University,Ningbo 315211,China);Distribution of eight enzymes in various organs of Inimicus japonicus[J];Marine Sciences;2010-10
9 HE Gang1,2,HE Li1,FANG Chun-lin2,XU Ying-fang1,WANG Deng-qiang1,FEI Chun-ping3(1.Fishery Rresources and Environment Key Field Station of Upper-middle Reaches of the Yangtze River,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Ministry of Agriculture,Wuhan 430223,China; 2.Fisheries Research Institute of Jiangxi Province,Nanchang 330039,China; 3.Jiangxi Jinguiwang Business Co.,Ltd,Jiangxi Shangrao 334400,China);Morphologic characters of hybrid(Chinemys reevesii♀×Ocadia sinensis♂) and comparison with their parents[J];Journal of Nanchang University(Natural Science);2011-06
10 ZHU Chun-hua, LI Guang-li, HUANG Rong-lian(Fisheries College,Zhanjiang Ocean University, Zhanjiang 524025,China);A STUDY ON ISOZYMES DURING EARLY ONTOGENY OF PENAEUS VANNAMEI BOONE[J];Journal of Tropical Oceanography;2004-01
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 CHU SINLUO CHEN YINBUI (Kunming Institute of Zoology, Aeademia Sinica);A NEW BLIND COBITID FISH (PISCES, CYPRINIFORMES) FROM SUBTERRANEAN WATERS IN YUNNAN, CHINA[J];Acta Zoologica Sinica;1979-03
2 WANG Shi Qiang\ \ ZHOU Zeng Quan\ \ GAO Tian Li (College of Life Sciences, Peking University, Beijing 100871, China);FEATURES OF THE CARDIOVASCULAR FUNCTIOIN IN HIBERNATING MAMMALIANS[J];ACTA ZOOLOGICA SINICA;1997-02
3 JI Qing;LI Zhongyun(Department of Biology,Xuzhou Teachers College Xuzhou 221009) YANG Ying;ZHU Jingan;TANG Jiguang(Department of Laboratory,Xuzhou City Hospital Xuzhou 221003);RELATIONSHIP BETWEEN SOME SERUM HORMONES AND GLUCOSE METABOLISM IN BUFO GARGARIZANS DURING PRE-HIBERNATION,HIBERNATION AND POST-HIBERNATION[J];CHINESE JOURNAL OF ZOOLOGY;1995-02
4 Liang Qanjin Liu Jia He Dacheng (The Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of State Education of China. Beijing Normal University, 100875, Beijing, China);THE EFFECTS OF CYCLOPHOSPHAMIDE ON ESTERASE ISOZYMES IN SEVERAL TISSUES OF MICE (Mus musculus albula) AND THE SUPPRESSION OF FETUS PROTECTION PILL (SHOUTAI WAN) AGAINST THEM[J];Journal of Beijing Normal University(Natural Science);2001-04
5 ZHU Xin ping, CHEN Yong le, WEI Cheng qing, LIU Yi hui, LU Xiao dan (Key Laboratory of Tropical and Subtropical Fishes Breeding and Culture, Agriculture Ministry Pearl River Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Guang;Growth of one-year old Asian yellow pond turtle (Mauremys mutica Cantor)[J];Journal of Dalian Fisheries University;2001-01
6 ZHANG Ying 1, DONG Shi 1, WANG Qian 2, SUN Zhen-rong 2 (1.Department of Chemistry and Biology, Tianjin Normal University,Tianjin 300074, China; 2. Faculty of Fisheries Science, Tianjin Agricultural College, Tianjin 300384, China);The isozyme genetic structures in large icefish (Protosalanx hyalocranius) and Taihu Lake icefish (Neosalanx taihuensis)[J];Journal of Dalian Fisheries University;2005-02
7 HE Li~1,WANG Xue-guang~2,CHENG Qing-chun~3,XIANG Jun-zu~3(1.Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Jingzhou,Hubei 434000;2.Bureau of Fisheries,Ministry of Agriculture of the Pepoles'Republic of China,Beijing 100026;3.Bureau of Aquatic Product and Store Farm of Longshan County,Longshan,Hunan 416800);Morphological Description on Triptophysa xiangxiensis[J];Freshwater Fisheries;2006-04
8 Yang Jun-xing Chu Xin-luo(Kunming Institute of Zoology, Aczdemia Sinica);DIFFERENTIATION OF THREE LOACHES OF THE GENUS TRIPLOPHYSA IN NANPAN BASIN, YUNNAN (PISCES: COBITIDAE)[J];Acta Zootaxonomica Sinica;1990-03
9 WANG Da Zhong LI De Jun(Department of Biology,Zunyi Medical College,Zunyi,Guizhou 563003);TWO NEW SPECIES OF THE GENUS TRIPLOPHYSA FROM GUIZHOU,CHINA (CYPRINIFORMES:COBITIDAE)[J];Acta Zootaxonomica Sinica;2001-01
10 Huang Shengmin, Li Shaolan, Yang Lianxi, Ye Wenjuan, Zhang Hanyun, Hu Jun (Kunming Institute of Zoology, Academia Sinica) Zhou Zhengwei, Zeng Libo (Shanghai Institute of Entomology, Academia Sinica);STUDIES ON ESTERASE ISOENZYMES OF DIFFERENT ORGANS IN BOMBYX MORI[J];Zoological Research;1983-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved