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《Acta Zootaxonomica Sinica》 2004-02
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A STUDY ON DNA POLYMORPHISMS BASED ON RAPD AMONG FOUR SPECIES IN ARCYPTERIDAE

ZHANG Jian-Zhen, MA En-Bo, GUO Ya-Ping, CHEN Dong-Hua,WANG Yan College of Life Science and Technology, Shanxi University, Taiyuan 030006,China  
Random Amplified Polymorphic DNA (RAPD)markers were applied to analyze genetic relationships among four species of Arcypteridae:Ceracris hoffmanni Uvrov,C. fasciata fsaciata (Br.-W.), Arcyptera coreana Shiraki and Pararcyptera microptera meridionalis(Ikonn.). Genomic DNA of twenty-four individuals was respectively amplified with eleven oligonucleotide (10*!mer) primers which were previously selected from twenty-four random primers (10*!bp), and a total of 128 clear and reproducible RAPD bands were observed. The amplified DNA fragments of individual primer were 10-13, and their molecular size was 100-2*!000*!bp. The dendrogram based on Nei′s genetic distance of RAPD markers was constructed using UPGMA and Neighbor-Joining. The cluster analysis showed that the species of each genus first clustered respectively and 4 species of Acridoidea were divided into two branches. One is C.hoffmanni Uvrov and C.fasciata fasciata (Br.-W.) cluster, and the other is A. coreana Shiraki and P. microptera meridionalis(Ikonn.) cluster. Whose conclusion coincided with the results of morphology, and this implied that RAPD seemed suitable for analysis of genetic relationships among genera.
【Fund】: 国家自然科学基金 (30 0 70 1 1 2 30 1 70 61 2 );; 山西省自然科学基金资助项目 (991 0 96)
【CateGory Index】: Q969
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