DETECTION OF WATER POLLUTION BY MICRONUCLEI AND OTHER NUCLEAR ANOMALIES OF ERYTHROCYTES OF TADPOIES (BUFO BUFO AN DREW SI)
He Weishun Wang Ruifang (Laboratory of Animal Genetics and Evolution, Kunming Institute of Zoology, Academia Sinica)
These studies present a approach to analysis of microiiuclei (MN) and other nuclear anomalies (ONA) in erythrocytes of toad (Bufo bufo andrewsi) tadpoles, induced by exposure to polluted water of Daguan River. Tadpoles were exposed to polluted water of three different concentration (33.3% 50.0% and 100.0%) for 7 clays. In the control, total frequency of nuclear anomalies (including micronuclei) was 4.1‰;Total frequencies of ONA for the above mentioned experiment groups were 8‰, 10.8‰and 15.8‰ respectively. The frequencies of MN and ONA in erythrocytes of tadpole, in various groups, showed distinct dose dependent increases.Our results shown that MN and ONA in erythrocytes of toad tadpole is useful index for detecting genotoxic compounds in the aquatic environment.