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《Zoological Research》 2002-01
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Cloning and Sequence of Nicotinic Acetylcholine Receptor α Subunit from Chilo suppressalis

HAN Zhao-jiu,HAN Zhao-jun 1 (Key Laboratory of Monitoring and Management of Plant Diseases and Pests,Ministry of Agriculture, Nanjing Agricultural University,Nanjing 210095,China)  
Nicotinic acetylcholine receptors (nAChRs) play a significant role in excitatory synaptic transmission in insects and are the target for chloronicotinyl and nereistoxin insecticides.In recent years,Chilo suppressalis,an economically important pest of rice,developed high resistance against monosultap,a nereistoxin insecticide acting on nAChR.In order to reveal the hypothesized target insensitive mechanism,studies on the molecular property of nAChR from Chilo suppressalis are required.In this study,the full length cDNA of nAChR α subunit from this pest was cloned by RT-PCR.Sequence analysis shows that it is a novel nAChR α subunit,which was named as Cs α 1(Genbank accession No.AF418987).It contains 1?997?bp nucleotides and involves an open reading frame (ORF) encoding a mature protein of 509 amino acids excluding a signal peptide of 24 amino acids.The deduced amino acid sequence was 52%-94% identical to the reported insect nAChR genes.
【Fund】: 国家重点基础研究与发展规划项目 (J2 0 0 0 0 16 2 0 7);; 国家自然科学基金资助项目 ( 30 0 70 5 0 2 )~~
【CateGory Index】: Q966
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