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《Zoological Research》 2002-05
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Cloning and Sequencing of Two Acetylcholinesterase cDNA Fragments from Cotton Aphid,Aphis gossypii Glover

LI Fei,HAN Zhao-jun 1 (Key Laboratory of Monitoring and Management of Plant Diseases and Pests,Ministry of Agricultural, Nanjing Agricultural University,Nanjing 210095,China)  
Two acetylcholinesterase (AChE) genes cDNA fragments,Ag.acel and Ag.ace2,have been cloned from cotton aphid,Aphis gossypii Glover using degenerate primers with RT-PCR technique.Ag.acel gene cDNA fragment is of 282?bp encoding 94 amino acids,and Ag.ace2 gene cDNA fragment is of 264?bp encoding 88 amino acids.Both two putative AChE genes cDNA fragments share numerous similarities with those cloned from other insects.This is the first report of two AChE cDNA fragment sequences in the insect species,which provided the direct evidence of multiple AChE existence in insects.
【Fund】: 国家自然科学基金资助项目 ( 39970 488);; 国家重点基础研究与发展规划项目 (J2 0 0 0 16 2 0 7)~~
【CateGory Index】: Q969.367.3;Q963
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【References】
China Proceedings of conference Full-text Database 1 Hits
1 MA Hui~1 GU Chun-bo~2 ZHAO Ming~1 ZHOU yu~1 XIA Xiao-ming~1 XUE Chao~1 (1.Cotton Research Center,Shandong Academy of Agricultural Science,Ji'nan,Shandong Province,250100,China; 2.Zaozhuang Entry-exit Inspection And Quarantine,Zaozhuang,Shandong Province 277102,China);Research Progress in Resistance of Cotton Aphids[Aphis gossypii(Glover)]to Insecticides[A];[C];2008
【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 WANG Jian Jun 1,2 , HAN Zhao Jun 1, WANG Yin Chang 1 (1 Key Laboratory of Monitoring and Management of Plant Diseases and Insects of Agricultural Ministry, Nanjing Agriculture University,Nanjing 210095, China;2 Department of Plant Protection,;cDNA fragment clone and sequence analysis of acetylcholinesterase gene in the diamondback moth, Plutella xylostella (L.)[J];Acta Entomologica Sinica;2002-04
2 LI Fei, HAN Zhao-Jun , TANG Bo (Key Laboratory of Monitoring and Management of Plant Diseases and Pests, the Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China);Insensitivity of acetylcholinesterase and increased activity of esterase in the resistant cotton aphid, Aphis gossypii Glover[J];Acta Entomologica Sinica;2003-05
3 WANG Dun~ 1, 2 , TANG Zhen-Hua~ 2* , SHANG Jin-Yan~1, ZHANG Chuan-Xi~1 (1. Institute of Applied Entomology, Zhejiang University, Hangzhou 310029, China; 2. Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China);Advances in the studies of acetylcholinesterase genes from insects[J];Acta Entomologica Sinica;2006-03
4 ZHANG Ling-min1, WU Ming-wei2 (1. Department of Parasitology,Medical College, Jinan University, Guangzhou 510632, China; 2. Cancer Institute, Sun Yat-sen University of Medical Science,Guangzhou 510060, China);CLONE AND SEQUENCE ANALYSIS OF THE ACETYLCHOLINESTERASE GENE FRAGMENT FROM AEDES ALBOPICTUS[J];Chinese Journal of Parasitic Disease Control;2003-01
5 WU Ming\|wei\+1, ZHANG Ling\|min\+1, HUANG Jiong\|lie\+2, ZHOU Guo\|li\+2, WU Yu\+2, ZHAO Shuang\|xing\+1 (1\ Department of Parasitology, Medical College, Jinan University, Guangzhou 510632; 2\ Department of Parasitology, Sun Yat\|Sen University of Me;Aedes albopictus: Cloning and Identification of the Acetylcholinesterase Gene Fragment from the Mosquito[J];Chinese Journal of Parasitology and Parasitic Diseases;2001-04
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Liu Zewen et al (Nanjing Agric Univ,Nanjing,Jiangsu 210095);Susceptibilities of Brown Planthopper and White Backed Planthopper from Anqing[J];Journal of Anhui Agricultural Sciences;2002-04
2 MENG Qing-fan, LIN Xiang-you, LI Wei, FENG Yun- TENG Li-rong (College of Life Science, Jilin University, Changchun; 130012, China);Comparison of Amino Acid Contents between Cultivated Cordyceps militaris and Wild Cordyceps sinensis[J];Amino Acids & Biotic Resources;2005-02
3 REN Xiao-Xia HAN Zhao-Jun WANG Yin-Chang (Key Lab of Monitoring and Management of Plant Disease and Insects, Ministry of Agriculture,Nanjing Agricultural University, Nanjing 210095, China);CLONING AND SEQUENCE ANALYSIS OF A cDNA FRAGMENT ENCODING ACETYLCHOLINESTERASE IN COTTON BOLLWORM (HELICOVERPA ARMIGERA)[J];Acta Zoologica Sinica;2002-01
4 XIE Jia yan 1, HE Feng qin 1, LI Mei 1, ZHANG Wei 1, FENG Guo lei 1, DONG Jian xin 2 (1 State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology,The Chinese Academy of Sciences, Beijing 100080, Ch;Relationship between esterase activity and insecticide resistance of cotton aphid in different areas[J];Journal of Agricultural University of Hebei;2001-03
5 ZHANG Hong ying,CHI Guo tong,ZHANG Jin lin (College of Plant Protection, Agricultural University of Hebei, Baoding 071001, China);The progress of detoxification enzyme systems and resistance of insecticide[J];Journal of Agricultural University of Hebei;2002-S1
6 Wu Fu-an Tian Li-dao (The Sericultural Research Institute,Chinese Academy of Agricultural Sciences,ZhenJiang 212018,China);STUDIES ON CONTROLLING MULBERRY RED SPIDER-MITE (TETRANYCHUS CINNABARINUS BOISDUVAL)BY SPRAYING AMITRAZ[J];Acta Sericologica Sinica;1993-02
7 TAO Shiqiang~1 WU Fuan~(2*) YU Maode~3 WANG Jun~1 CHENG Jialing~2(~1 College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China; ~2 Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China; ~3 College of Sericulture and Biotechnology, Southwest Agricultural University, Chongqing 400716, China);Study on Laboratory Population Parameters of Carmine Spider Mite(Tetranychus cinnabarinus) Infesting Mulberry YU71-1[J];Acta Sericologica Sinica;2005-03
8 WU Fu-An~1 YU Mao-De~2 WANG Xing-Ke~1 CHENG Jia-Ling~1 ~1 The Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang Jiangsu 212018,China; ~2College of Sericulture and Biotechnology of Southwest University,Chongqing 400716,China;Statistical Inference of Controlling Effects of Propargite on Carmine Spider Mite (Tetranychus cinnabarinus) in Mulberry (Morus L.) Garden by Bootstrap Method[J];Science of Sericulture;2006-04
9 HAN Zhao-jiu,HAN Zhao-jun 1 (Key Laboratory of Monitoring and Management of Plant Diseases and Pests,Ministry of Agriculture, Nanjing Agricultural University,Nanjing 210095,China);Cloning and Sequence of Nicotinic Acetylcholine Receptor α Subunit from Chilo suppressalis[J];Zoological Research;2002-01
10 DING Wei 1,Eli SHAAYA 2,WANG Jin jun 1,ZHAO Zhi mo 1,GAO Fei 1 (1 Department of Plant Protection,Southwest Agricultural University,Key Laboratory of Entomology and Pest Control Engineering,Ministry of Agricultural and Chongqing City,Chongqing;Acute Lethal Effect of Two Insect Growth Regulators on Liposcelis entomophila (Psocoptera:Liposcelididae)[J];Zoological Research;2002-02
【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 HE Heng-guo1,2,ZHAO Zhi-mo1,WANG Jin-jun1*(1Key Laboratory of Entomology and Pest Control Engineering,College of Plant Protection,Southwest University,Chongqing 400716 China;2College of Life Science,China West Normal University,Nanchong,Sichuan 637002 China);Biochemical and toxicological characterizations of Na~+-K~+-ATPase in citrus red mite,Panonychus citri McGregor (Acari: Tetranychidae)[J];Journal of Fruit Science;2011-01
2 FANG Ye1,LIN Tong2(1.Hepai State Forest Farm of Jiangmen City,Jiangmen 529431,China; 2.College of Forestry,South China Agricultural University,Guangzhou 510642,China);Effect of chloramine phosphorus on Biston marginata AChE activity and its virulence against Myzus persicae[J];Hunan Forestry Science & Technology;2012-04
3 HUANG Xiao,TENG Yun,TAO Ke,HE Yan,GENG Yu-cong,HOU Tai-ping(College of Life Science,Sichuan University,Chengdu 610064);Inhibitory of Acetylcholinesterase in Silkworm by Natural Product Extracts[J];Sichuan Journal of Zoology;2008-03
4 PENG Xia,TAO Ke,TENG Yun,HUANG Xiao,HOU Tai-Ping(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education,College of Life Sciences,Sichuan University,Chengdu 610064,China);Purification and characterization of acetylcholinesterase,a kind of pesticide target[J];Journal of Sichuan University(Natural Science Edition);2008-01
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