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《Zoological Research》 2003-04
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Phylogenetic Relationships of Some Species and Genera in Megophryids Inferred from Partial Sequences of Mitochondrial 12S and 16S rRNA Genes

JIANG Jian ping 1,3 ,YUAN Fu rong 2,XIE Feng 1,ZHENG Zhong hua 1 (1.Chengdu Institute of Biology,the Chinese Academy of Sciences,Chengdu 610041,China; 2.Department of Physiology,the University of Texas Health Science Center at San Antonio,TX 78229-3900,USA)  
Fragments of mitochondrial 12 S and 16 S rRNA genes of 17 individuals were sequenced,including 8 species (subspecies) of 2 genera Megophrys and Brachytarsophrys of Megophrinae,and 3 species used as outgroup including the genera Bombia , Oreolalax and Scutiger .Results pairwised by Clustal W show that there are 949?bp in length with 320 variable sites including 206 parsimony sites.The average contents of T,C,A,and G are 25 6%,24 0%,29 8%,and 20 6%,respectively,and the ratio of transition to transvertion is 1 3.The phylogenetic relationships of these taxa were analyzed using programs of Neighbor Joining and Maximum Parsimony.The results show that the ingroup are clustered together as one clade,of which 3 individuals of M glandulosa were clustered together (BSP=100%) and firstly diverged from the other taxa (BSP=100%),3 individuals of B carinensis were clustered together (BSP=100%) as sister group of M shapingensis ,the rest 5 taxa formed together and included two groups,one consisting of two populations of M minor brachykolos from Longsheng of Guangxi and Hong Kong,the other containing M omeimontis,M minor minor,M caudoprocta and M sp from Wulong of Chongqing,the later two species are closely related.These results support M brachykolos as a valid species rather than a subspecies of M minor ,and suggest that M glandulosa,M shapingensis and B carinensis may belong to three different subgenera or genera.
【Fund】: 国家自然科学基金资助项目 ( 3 0 0 0 0 0 18) ;; 中国科学院生物科学与技术特别支持费资助项目 (STZ -0 1-19) ;; 四川省科技 厅重点项目 ;; 香港野生动物保护基金会有限公司 (WCF)项目
【CateGory Index】: Q953
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