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《Zoological Research》 2006-03
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Isolation, Culture and Biological Characteristics of Bovine Mammary Epithelial Cells

DUO Shu-guang, WU Ying-ji, LUO Fen-hua, BOU Shorgan (Key Laboratory of China Education Ministry for Mammal Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010021, China)  
Bovine mammary epithelial cells were isolated and purified by using collagenase digestion of the mammary gland tissue for the primary cell culture, and subsequently using trypsin-selected digestion of the cultured cells for cell purification. Morphological observation revealed that the cultured cells possessed the typical character of epithelial cells. Karyotyping analysis showed the normal chromosome number in cultured cells. The tissue-specific expression of cytokeratin 5 and 8 genes in mammary epithelial cells was identified by immunofluorescent cytochemical staining. The transcription of the beta-casein gene was detected by RT-PCR, when the purified cells were induced with insulin, hydrocortisone and prolactin in the culture medium without serum. The above results indicate that the purified cells are mammary epithelial cells, which can transcript beta-casein mRNA in the induction condition.
【Fund】: 内蒙古大学特聘教授科研启动费资助项目(203059);; 内蒙古自治区自然科学基金(200408020402);; 国家高科技项目(2005AA206110)子课题资助项目
【CateGory Index】: Q813.11
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【References】
Chinese Journal Full-text Database 10 Hits
1 FU Guo-wen et al(College of Animal Science and Technology,Northwest A & F University of Sciences and Technology University,Yangling,Shaanxi 712100);Goat Mammary Epithelial Cells Cultured by Digestion Method[J];Journal of Anhui Agricultural Sciences;2007-24
2 ZHUANG Xin-jie et al(Animal Reproduction Institute,Guangxi University,Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization,Nanning,Guangxi 530005);Isolation,Cuture and Biological Characteristic Analysis of Stromal and Glandular Epithelial Cells of Buffalo(Bubalus bubalis) Endometrium[J];Journal of Anhui Agricultural Sciences;2009-18
3 BI Lin et al(College of Life Science and Technology,Southwest University for Nationalities,Chengdu,Sichuan 610041);Research Progress of Primary Culture Method of Mice Mammary Epithelial Cells[J];Journal of Anhui Agricultural Sciences;2010-07
4 TIAN Tian et al (College of Life Science and Technology,Southwest University for Nationalities,Chengdu,Sichuan 610041);Optimization of Parameters of Exogenous Gene Transfection of Yak Mammary Epithelial Cells in vitro Mediated by Liposome[J];Journal of Anhui Agricultural Sciences;2010-09
5 CHEN Jianhui,GAO Xuejun,LI Qingzhang (Key Laboratory of Dairy Sciences,Ministry of Education,College of Food,Northeast Agricultural University,Harbin 150030,China);Research progress of culture technique on diary cow mammary gland epithelial cells in vitro[J];Journal of Northeast Agricultural University;2009-07
6 YAN Jing,HE Xiao-ying,PAN Chun-liu,LAN Zhi-gang,ZHANG Yong (College of Veterinary Medicine,Institute of Bio-engineering,Northwest A & F University,Yangling,Shaanxi,712100,China);Construction of Eukaryotic Expression Vector pEGFP-hTERT and the Optimization of Scheme on Bovine Mammary Cell Transfection[J];Progress in Veterinary Medicine;2009-05
7 GONG Chao,WANG Ling-yun,ZHOU Huan-min(College Of Life Sciences,Inner Mongolia Agricultural University,Huhhot,Inner Mongolia,010018,China);Study on Culture of Bovine Mammary Epithelial Cells in Vitro[J];Progress in Veterinary Medicine;2011-07
8 LIN Jie;WANG Xu-rong;LI Jian-xi;WANG Xiao-wu;YANG Zhi-qiang;Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province,Key Laboratory of Veterinary Pharmaceutics Discovery,Ministry of Agriculture,Lanzhou Institute of Husbandry and Pharmaceutical Science of CAAS;;Research Progress on Primary Culture,Purification and Identification Technique of Bovine Mammary Epithelial Cells[J];China Animal Husbandry & Veterinary Medicine;2015-08
9 LI Xi-yan,WANG Jia-qi,WEI Hong-yang,BU Deng-pan,HU Han,ZHOU Ling-yun(State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China);Bovine Mammary Epithelial Cells Culture Methods in vitro and Its Application[J];Acta Agriculturae Boreali-Sinica;2010-S2
10 LI Zhong-qiu(Animal Husbandry Institute of Heilongjiang Academy of Agricultural Sciences,Harbin 150086);Progress of the Bovine Mammary Epithelial Cells[J];Heilongjiang Agricultural Sciences;2008-05
【Citations】
Chinese Journal Full-text Database 5 Hits
1 WT5BZ]Li Zhen Wang Ying Liu Huili Zhang Defu [WT5BX](Animal Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences, Laboratory of Animal Genetic Engineering,Shanghai Municipal Key Laboratory of Agro genetics a;STUDY ON ISOLATION AND CULTURE OF BOVINE [WT4HZ]MAMMARY EPITHELIAL CELLS[J];ACTA AGRICUL TURAE SHANGHAI;2000-03
2 Xu Manni 1,2 Li Shuguang 1 Zhao Jianyang 2 Cheng Guoxiang 2 ( 1 Dept of Preventive Medicine College of Medicine Tong Ji University, Shanghai 200331; 2 Transgenic Research Center of Shanghai, Shanghai 201203);The Detection Method of Mammary Gland Bioreactor Expression Vector[J];Biotechnology Information;2003-05
3 OUYANG Wu qing,QIAN Ju fen (College of Animal Sciences and Technology,Northwestern Sci Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China);Establishment of culture system in goat mammary epithelial cell[J];Journal of Northwest Sci-Tech University of Agriculture and Forestry;2003-03
4 ZHENG Yue-mao,PENG Xin-rong,XU Yong-ping,SHI Yu-qiang,ZHANG Yong(Institute of Bio-Engineering,Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China);Study on appearance of goat mammary epithelialcells which cultured in vitro[J];Journal of Northwest Sci-Tech University of Agriculture and Forestry;2004-03
5 HOU Ying~(1, 2), TAN Li-Xin~1, LI Wen-Rong~1, NIU Zhi-Gang~1,GUO Zhi-Qin~1(1. Key Laboratory of Livestock Reproduction and Breeding Biotechnology of Ministry of Agriculture,Xinjiang Academy of Animal Science,Urumqi 830000, China;2.College of Life Science and Technology,Xinjiang University,Urumqi 830046, China);A Novel Method for Increasing the Number of Metaphase Cells Cultured in vitro[J];Hereditas(Beijing);2005-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Gao Yajun, Tang Shuang, Sun Sujun, Zhang YongInstitute of Bio-Engineering, Northwest A & F University, Yangling, Shanxi 712100, China;In Vitro Culture of Mammary Epithelial Cells in Goat[J];Anhui Agricultural Science Bulletin;2005-02
2 FU Guo-wen et al(College of Animal Science and Technology,Northwest A & F University of Sciences and Technology University,Yangling,Shaanxi 712100);Goat Mammary Epithelial Cells Cultured by Digestion Method[J];Journal of Anhui Agricultural Sciences;2007-24
3 WU Juan et al(Department of Pathology,College of Animal Science and Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010018);Primary Culture of Bovine Mammary Epithelial Cells[J];Journal of Anhui Agricultural Sciences;2009-06
4 BI Lin et al(College of Life Science and Technology,Southwest University for Nationalities,Chengdu,Sichuan 610041);Research Progress of Primary Culture Method of Mice Mammary Epithelial Cells[J];Journal of Anhui Agricultural Sciences;2010-07
5 TONG Huili,YIN Deyun,LI Qingzhang,GAO Xuejun(Key Laboratory of Dairy Science,Ministry of Education,Northeast Agricultural University,Harbin 150030,China);Establishment of dairy goat mammary gland epithelial cell line[J];Journal of Northeast Agricultural University;2008-08
6 CHEN Jianhui,GAO Xuejun,LI Qingzhang (Key Laboratory of Dairy Sciences,Ministry of Education,College of Food,Northeast Agricultural University,Harbin 150030,China);Research progress of culture technique on diary cow mammary gland epithelial cells in vitro[J];Journal of Northeast Agricultural University;2009-07
7 YAN Jing,HE Xiao-ying,PAN Chun-liu,LAN Zhi-gang,ZHANG Yong (College of Veterinary Medicine,Institute of Bio-engineering,Northwest A & F University,Yangling,Shaanxi,712100,China);Construction of Eukaryotic Expression Vector pEGFP-hTERT and the Optimization of Scheme on Bovine Mammary Cell Transfection[J];Progress in Veterinary Medicine;2009-05
8 GONG Chao,WANG Ling-yun,ZHOU Huan-min(College Of Life Sciences,Inner Mongolia Agricultural University,Huhhot,Inner Mongolia,010018,China);Study on Culture of Bovine Mammary Epithelial Cells in Vitro[J];Progress in Veterinary Medicine;2011-07
9 LIU Yu,ZHU Yi-wen,XU Miao,HUANG Ying(Key Laboratory of Medical Embryo and Molecular Biology of the Ministry of Health,Shanghai Key Laboratory of Embryo and Reproduction Engineering,Shanghai Institute of Medical Genetics,Children's Hospital of Shanghai,Shanghai 200040,China);Detection of the Integration of Human Transferrin Gene on Chromosomes in Transgenic Cloned Cows[J];China Animal Husbandry & Veterinary Medicine;2012-02
10 LIU Chang,ZHAO Feng,LI Qing-zhang(Key Laboratory of Dairy Science,Ministry of Education,Northeast Agricultural University,Harbin 150030,China);Influence of Different Hormone and Cytokine Related Lactation on the Proliferation of Mammary Epithelial Cells of Dairy Cow[J];China Animal Husbandry & Veterinary Medicine;2012-05
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Gao Yajun, Tang Shuang, Sun Sujun, Zhang YongInstitute of Bio-Engineering, Northwest A & F University, Yangling, Shanxi 712100, China;In Vitro Culture of Mammary Epithelial Cells in Goat[J];Anhui Agricultural Science Bulletin;2005-02
2 Yang Keli et al (Hefei Shushan Cow Farm,Hefei 230031);The Experiment in the Isolution of Cow Mastitis Pathopen[J];JOURNAL OF ANHUI AGRICULTURAL SCIENCES;2000-05
3 QI Yong-hua et al(College of Animal Science,Henan Institute of Science and Technology,Xinxiang,Henan 453003);Isolation,Identification & Drug Sensitivity Test of the Pathogenic Bacteria of Cow Mastitis[J];Journal of Anhui Agricultural Sciences;2006-14
4 GUAN Xiao-bin et al(The College of Animal Science and Technology,Sichuan Agricultural University,Yaan,Sichuan 625014);Construction of the Mammary Gland-specific Expression Vector by Bovine αS1-casein Gene[J];Journal of Anhui Agricultural Sciences;2006-23
5 FU Guo-wen et al(College of Animal Science and Technology,Northwest A & F University of Sciences and Technology University,Yangling,Shaanxi 712100);Goat Mammary Epithelial Cells Cultured by Digestion Method[J];Journal of Anhui Agricultural Sciences;2007-24
6 HU Chuan-huo et al(College of Animal Science and Technology,Guangxi University,Nanning,Guangxi 530005);Effect of Lactoferrin on Cryopreservation Effect of Bull Semen[J];Journal of Anhui Agricultural Sciences;2007-36
7 WU Juan et al(Department of Pathology,College of Animal Science and Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010018);Primary Culture of Bovine Mammary Epithelial Cells[J];Journal of Anhui Agricultural Sciences;2009-06
8 BI Lin et al(College of Life Science and Technology,Southwest University for Nationalities,Chengdu,Sichuan 610041);Research Progress of Primary Culture Method of Mice Mammary Epithelial Cells[J];Journal of Anhui Agricultural Sciences;2010-07
9 SUN Xu-qun, ZHAO Xin-ming , YANG Xu (Dept. of Pharmacy,The First Hospital Affiliated to Anhui Medicine University,Anhui Hefei 230022,China);Experimental Research on the Cholagogic Action of Gargenoside[J];Journal of Anhui Traditional Chinese Medical College;2004-05
10 HUI Zhou-Guang, LI Ye-Xiong, YANG Wei-Zhi, YU Zi-HaoDepartment of Radiation Oncology, Cancer Hospital (Institute), Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, P.R.China;Abrogation Effect of UCN-01 on Radiation-induced G_2 Phase Arrest of Tumor Cells and Its Mechanism[J];Chinese Journal of Cancer;2005-01
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 LI Rui-ming1, LING Ze-ji1, SONG Xiao-bai1, QIN Jin1, XIE Ying-xue1, SHI Yu-jing2 1. College of Animal Science and Technology, Guangxi University, Nanning 530004, China; 2. Veterinary College, Yangzhou University, Yangzhou 225009, China;Immunohistochemical Localization and Receptor Expression of Gonadotropin-Releasing Hormone in Pituitary of Guangxi Swamp Buffaloes[J];畜牧与饲料科学(英文版);2010-05
2 LIU Wei et al (Center of Animal Embryo Engineering,Qingdao Agricultural University,Qingdao,Shandong 266109);Isolation and Culture and Molecular Biological Identification on Cows Mammary Epithelial Cells[J];Journal of Anhui Agricultural Sciences;2010-06
3 BI Lin et al(College of Life Science and Technology,Southwest University for Nationalities,Chengdu,Sichuan 610041);Research Progress of Primary Culture Method of Mice Mammary Epithelial Cells[J];Journal of Anhui Agricultural Sciences;2010-07
4 TIAN Tian et al (College of Life Science and Technology,Southwest University for Nationalities,Chengdu,Sichuan 610041);Optimization of Parameters of Exogenous Gene Transfection of Yak Mammary Epithelial Cells in vitro Mediated by Liposome[J];Journal of Anhui Agricultural Sciences;2010-09
5 QIONG Da et al(Agriculture and Animal Husbandry College of Tibet,Linzhi,Tibet 860000);Correlation and Regression Analysis of the Body Weight and Body Size of Adult Female Yak in Naqu[J];Journal of Anhui Agricultural Sciences;2011-16
6 WU Qing-xia et al(College of Animal Science,Tibet Agricultural and Animal Husbandry College,Linzhi,Tibet 860000);Comparison on In-Vitro Culture Methods of Yak Endometrial Gland Epithelial Cells[J];Journal of Anhui Agricultural Sciences;2011-18
7 GAN Yuan et al(Traditional Chinese Medicine Research Institute,Southwest Jiaotong University,Chengdu,Sichuan 610031);Investigation of Characteristics for Yield and Quality of Curcuma phaeocaulis Val.from Sichuan Province[J];Journal of Anhui Agricultural Sciences;2011-22
8 ZHOU Miaomiao WU Yueming LIU Hongyun ZHAO Ke LIU Jianxin(Institute of Dairy Science,Ministry of Education Key Laboratory of Molecular Animal Nutrition, Zhejiang University,Hangzhou 310029,China);Role of Oligopeptide Transporter 2 in Bovine Mammary Gland Phenylalanine Dipeptide Uptake[J];Chinese Journal of Animal Nutrition;2011-08
9 MA Yanfen1 ZHAO Jun2 GAO Min1 LU Dexun1(1.Animal Nutrition Institute,Inner Mongolia Academy of Animal Science,Huhhot 010031,China; 2.Xing An Meng Jiu Zhou Da Di Feed Co.Ltd.,Wulhot 137400,China);Off-Flavor in Raw Milk and Its Control Measures[J];Chinese Journal of Animal Nutrition;2012-01
10 GONG Chao,WANG Ling-yun,ZHOU Huan-min(College Of Life Sciences,Inner Mongolia Agricultural University,Huhhot,Inner Mongolia,010018,China);Study on Culture of Bovine Mammary Epithelial Cells in Vitro[J];Progress in Veterinary Medicine;2011-07
China Proceedings of conference Full-text Database 1 Hits
1 Chen Xinping;Xu Bangfa;Luo Fenhua;Zhang Pei;Zhou Hongtao;Cai Junhong;Wu Yingji;Fu Shengmiao;Postgraduate Program College of Agriculture,Hainan University;Department of Medical Examination,Hainan Provincial People's Hospital,Hainan Provincial Key Laboratory for Cell and Molecular Genetic Translational Medicine;The Key Laboratory for Mammalian Reproductive Biology and Biotechnology,Ministry of Education,Inner Mongolia University;;The Preliminary Identification of isolation and purification for Spermatogonial Stem Cells of Macaca Fascicularis[A];[C];2015
【Secondary Citations】
Chinese Journal Full-text Database 8 Hits
1 LIU Ji-Long;WANG Min-Kang; LI Jin-Song;SUN Qing-Yuan;XU Zhi CHEN Da-Yuan(State Key Laboratory of Reproductive Biology, Institute of Zoology, The Chinese Academy of Science, Bijing 100080, China)(Tianjin Husbandry & Veterinary Institute, Tianjin 300112;CULTURE OF BOVINE EAR FIBROBLASTS[J];ACTA ZOOLOGICA SINICA;1999-04
2 Zhang Kezhong; Chong Song; Chen Li; Lu Daru; Xue Jinglun; Qiu Xinfang(State key Laboratory of Genetic Engineering, Fudan University);Preliminary Study on the Expression of Mammary Gland Specific Expression Vector for Human Clotting Factor Ⅸ In Vitro and in Mouse Mammary Gland[J];JOURNAL OF FUDAN UNIVERSITY;1998-04
3 Li Zhen; Lin Aixing; Chen Yongfu; Xia Guoling (1 College of Animal Science and Technology, Shandong Agricultural Yniver sity, Taian Shandong 271018;2 National Labs for Agrobiotechnology and College of Biology, China Agricultural university, Beijing 100094;Induction of BME-L_1 to Secrect β-Casein and Its Expression of hEPO[J];JOURNAL OF AGRICULTURAL BIOTECHNOLOGY;1999-01
4 Li Zhen Chen Yongfu (National Lab for Agrobiotechnology, China Agricultural University, Beijing 100094);Problems Facing the Establishment of Cellular Model of Mammary Gland Bioreactors[J];JOURNAL OF AGRICULTURAL BIOTECHNOLOGY;1997-02
5 Yan Xijun Qiao Guilin Yue Junming Mei Zhuzhong Lai Liangxue YinZhen (Mill Vet Inst, Uni of Agri and Anim Sci, Changchun, 130000);CONSTRUCTION OF MAMMARY-SPECIFIC VECTOR OF HUMAN ERYTHROPOIETIN AND ESTABLISH OF ITS FEASIBILITY DETECTION METHOD[J];BIOTECHNOLOGY;1999-03
6 Wang yiping et al.:;The Methods of Preparing Early Metaphase Chromosomes and the Study on Their G-banding Patterns in Mouse Fibroblasts[J];Hereditas(Beijing);1989-01
7 CAO Xin,ZENG Yi-tao (Shanghai Institute of Medical Genetics,Shanghai Children′s Hospital,200040);The Tissue Specific Expression of Human Serum Albumin Gene Using Goat β-Casein Gene Promoter in the Mouse Tissues[J];Hereditas(Beijing);2001-06
8 Qiao Guilin Yan Xijun Yue Junming Mei Zhuzhong Lai Liangxue Huang Weimin Jia Bunian Yin Zhen (The Mil Vet Inst, Uni of Agri and Anim Sci, Changchun 130062);Construction of Mammary specific Vectors of Expressing Human Erythropoietin Gene and Its Transient Expression in Rat and Rabbit Mammary Gland[J];CHINESE JOURNAL OF VETERINARY SCIENCE;1999-01
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