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《ZOOLOGICAL RESEARCH》 1995-04
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MICROSATELLITE DNAS AND KINSHIP IDENTIFICATION OF GIANT PANDA

Zhang Yaping;Wang Wen;Su Bing(Laboratory of Cellular and Molecular Evolution,Kunming Institute of Zoology,the Chinese Academy of Sciences,Kunming 650223)Oliver A.Ryder (Zoological Society of San Diego,USA)Fang Zhiyong(National Ministry of Forestry of Chin  
We constructed a DNA library of 150-500 bp fragments from giant panda genomic DNA digested with DpnⅡ.Ten microsatellite DNA loci were isolated and characterized by screening the library with a (32)P-labelled (CA)(15) probe.The specific primers for each of the microsatellite DNA locus were synthesized and employed to amplify DNA samples isolated from 7 specimens of panda tissue and 6 specimens of hair.Nine of the 10 microsatellite DNA loci were polymorphic in the 13 samples.Inheritance patterns of alleles at these loci accurately agree with the well recorded pedigree.The polymorphism on these loci also clarified some previously unresolved paternity.These results imply the screened microsatellite DNA loci are useful makers for the identification of kinship among giant panda.
【Fund】: 美国圣地亚哥动物协会、中国科学院重点项目 云南省应用基础研究基金
【CateGory Index】: Q959
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