STUDIES ON FACTORS IN RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS
Chen Yongjiu Zhang Yaping (Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming 650223)
In this paper, we studied factors which may affect the results of random amplified polymorphic DNA. We found that different PCR amplifier, TaqDNA polymerase, and concentration of Mg 2+ , dNTP, BSA, gentin, and template DNA may result in different RAPD results. To make the RAPD data more repeative and comparitive, we recommand that those factors should be clearly stated in all the paper.