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《Acta Zoonutrimenta Sinica》 2001-02
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STUDY ON THE EFFECT OF CAROTENOID,SODIUM TAUROCHOLATE AND FREE FATTY ACIDS ON CAROTENOIDS UPTAKE BY INTESTINAL CELLS IN VITRO

CHEN Bo,ZHOU Guang-hong,LIU Qing(College of Animal Science,Nanjing Agricultural University,Nanjing 210095,China)  
The small intestinal mucous cells of yellow broilers were isolated and cultured with medium containing βC and lutein to determine the effect of carotenoid concentrations,sodium taurocholate and free fatty acids on the absorption of the two carotenoids .The results showed uptake of βC and lutein was concentration-dependent,and a constant uptake percentage of βC was observed.Addition of sodium taurocholate most significatly promoted the uptake of both carotenoids.The optimal concentration of sodium taurocholate for the uptake of βC and Lutein was 3mM and 10mM,respectively.When sodium taurocholate concentration exceeded 3mM,βC absorption decreased quickly.The absorption of both carotenoids most significantly increased with the addition of stearic and oleic acids,the optimal ratio of oleic/stearic for the absorption of βC and Lutein was 100:0 and 3:1,respectively.These experiments indicated that the some factor has different effects on the uptake of βC and Lutein;carotenoid uptake took place by passive diffusion;the process of diffusion could be modulated by intraluminal factors.
【CateGory Index】: Q489
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