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《Chinese Journal of Animal Nutrition》 2007-02
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Effect of Dietary Protein on the Activities of Digestive Enzymes of Topmouth Culter (Erythroculter Ilishaeformis Bleeker)

QIAN Xi1 WANG Gui-qin1,3 ZHOU Hong-qi1* CHEN Jian-ming2YE Jin-yun2 PAN Qian2 WANG You-hui2(1.College of Aqua-life Science and Technology,Shanghai Fisheries University,Shanghai 200090,China;2.Zhejiang Institute of Freshwater Fisheries,Huzhou 313001,China;3.College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China)  
The effects of dietary protein level and replacement of fishmeal by soybean meal on digestive enzyme activities of topmouth culter were investigated.In order to study the effect of dietary protein level on digestive enzyme activities of topmouth culter tested fish were fed on the same relativity of essential amino acid balance and isoenergetic experimental semi-purified diets with five dietary protein levels(31.04%、35.51%、40.89%、46.62% and 50.33%)respectively.And five isonitrigenous,isoenergetic and different relativity of essential amino acid balance experimental semi-purified diets with soybean protein replacing 0.0%,13.5%,27.0%,40.5%,54.0% of fish meal protein were formulated to determine the effect of replacement of fish meal by soybean meal on digestive enzyme activities of topmouth culter.The results showed that protease specific activity in the intestine and hepatopancreas were significantly influenced by dietary protein level.Protease specific activity in the intestine and hepatopancreas increased with increasing dietary protein level significantly(P0.05).Protease specific activity in 50.33% treatment was 171.26 U/mgPr and significantly higher(P0.05)than that of 31.04% dietary protein treatment(P0.05).Protease activities of the hepatopancreas in 40.89% and 46.62% treatments were 35.04 U/mgPr and 35.68 U/mgPr,which were significantly higher(P0.05)than those of 31.04% and 35.51% dietary protein treatments.Amylase and lipase specific activities in the intestine and hepatopancreas were not influenced by dietary protein level(P0.05).Protease specific activities in the intestine and hepatopancreas were significantly influenced by replacement of fishmeal by soybean meal.Protease specific activities in the intestine and hepatopancreas significantly were decreased with increasing replacement of fishmeal by soybean meal(P0.05).When the replacement level for fishmeal protein with soybean protein was 40.5%,protease specific activity in the intestine was 133.0 U/mgPr and significantly lower than that of control(P0.05).When the replacement level for fishmeal protein with soybean protein was 27.0%,protease specific activity in the hepatopancreas was 31.34 U/mgPr and significantly lower than that of control(P0.05).Amylase specific activity in the intestine was not influenced by replacement of fishmeal by soybean meal(P0.05),but amylase specific activity in the hepatopancreas was increased with increasing replacement of fishmeal by soybean meal(P0.05).Lipase specific activities in the intestine and hepatopancreas were not influenced by replacement of fishmeal by soybean meal(P0.05).
【Fund】: 上海市重点学科建设项目(Y1101);; 湖州市科技重点项目(2003GN04)
【CateGory Index】: S965.123
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