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《Chinese Journal of Animal Nutrition》 2008-06
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Effects of Achyranthes Bidentate Polysaccharide on the Proliferation of Lymphocytes and Concentrations of Cytokine in Peripheral Blood Mononuclear Cells of Piglets

CHEN Qing-hua LIU Zhu-ying HE Jian-hua(College of Animal Science and Technology,Hunan Agriculture University,Changsha 410128,China)  
The experiment was conducted to study the effects of diets added with achyranthesachyranthes bidentatebidentate polysaccharide(ABP) on the proliferation of lymphocytes and concentrations of cytokine in peripheral blood mononuclear cells of piglets and explore the immunity modulation mechanism of ABP.One hundred and twenty-eight 28-day-old three-line crossbred(Duroc×Landrace×Yorkshire) weaning piglets were randomly divided into 4 treatments and each treatment had 4 replicates with 8 piglets per replicate.The control group was fed with basal diet and the test groups(group Ⅰ~Ⅲ) were fed with basal diet added with 500,1 000,1 500 mg/kg of ABP respectively.On the 14th day and 28th day,4 piglets of each treatment were randomly picked out for blood-collection from thorax cavity vein,so as to determine lymphocyte proliferation of peripheral blood and the concentration of cytokine TNF,IL-1β,IL-2,IL-6.The results showed that: compared with control group,stimulation indices(SI) in groupⅠ,Ⅱ,Ⅲ were increased by 8.04%(P0.05) and 11.32%(P0.01),24.11%(P0.01) and 19.81%(P0.01),69.64%(P0.01) and 43.40%(P0.01) on the 14th day and 28th day respectively and there were significant differences in experimental groups(P0.05 or P0.01).On the 28th day,the value of SI was decreased compare with that on the 14th day.On the 14th day,compared with control group,the concentrations of cytokine TNF,IL-1β,IL-2,IL-6 in groupⅡand Ⅲ were significantly increased(P0.01).The concentration of TNF and IL-2 in groupⅠwas higher than that in control group(P0.05),and there were no significant difference for IL-1β and IL-6 between the control group and group Ⅰ(P0.05).On the 28th day,the concentrations of TNF,IL-1β and IL-6 in group Ⅰ,Ⅱ,Ⅲ were significantly higher than that in control group(P0.05 or P0.01);The concentration of IL-2 in group Ⅲ was significantly higher than that in control group(P0.05).However,there were no significant difference for IL-2 among group Ⅰ,Ⅱ and control group(P0.05).On the 28th day,the concentration of TNF and IL-1β was increased,and the concentration of IL-2 and IL-6 was decreased compared with that on the 14th day.Among the experimental groups,the proliferation of lymphocytes and concentrations of cytokine of group Ⅲ was significantly improved.In conclusion,ABP can improve lymphocyte proliferation and increase the concentration of cytokine TNF,IL-1β,IL-2,IL-6 in peripheral blood mononuclear cells of piglets.
【Fund】: 湖南省科技重大专项(生猪产业化关键技术研究与示范 项目编号:2007FJ1003)资助
【CateGory Index】: S828.5
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