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《Chinese Journal of Animal Nutrition》 2010-03
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Effects of Water Soluble Alfalfa and Astragalus Polysaccharide on Proliferation of Lymphocyte in Broilers

ZHANG Shiyuan1 XU Chunyan1 DONG Xiaofang2 TONG Jianming2 ZHANG Qi2 (1. Institute of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China; 2. State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China)  
In order to study the effects of water soluble alfalfa polysaccharide (WSAP) and astragalus polysaccharide (APS) on proliferation of peripheral blood and splenic lymphocyte in broilers,the proliferation was observed in peripheral blood and splenic lymphocyte treated with two polysaccharides at four concentrations respectively. The T and B lymphocytes proliferation were determined by MTT assay after culture for 48 h. The results showed that WSAP and APS at suitable dosages could significantly stimulate proliferation in peripheral blood and splenic lymphocyte,and WSAP at 20 μg/mL,APS at 100 μg/mL showed to the best advantage. In addition,the effects of APS on proliferation of peripheral blood and splenic lymphocyte in broilers were better than that of WSAP,but the effective additive amount of APS was more than that of WSAP. The proliferation of lymphocyte was likely to be one of mechanisms of WSAP and APS to enhance immunity,and the immunization of different plant polysaccharides was different.
【Fund】: 国家科技支撑计划课题(2006BAD12B05)
【CateGory Index】: S831.5
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