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Influence of Oxidized Fish Oil on Intestinal Mucosal Immune Response in Neonatal Piglets and the Use of Soybean Isoflavones as an Intervention Measure

HUANG Lin1,2 JIANG Zongyong1 LIN Yingcai1 ZHOU Guilian1 ZHENG Chuntian1 CHEN Fang1(1.Guangdong Public Laboratory of Animal Breeding and Nutrition,Key Laboratory of Animal Nutrition and Feed(South China),Ministry of Agriculture of China,State Key Laboratory of Livestock and Poultry Breeding,Institute of Animal Science,Guangdong Academy of Agricultural Sciences 510640,China;2.College of Animal Science,South China Agricultural University,Guangzhou 510640,China)  
This study was conducted to determine the effects of dietary oxidized fish oil(OFO) and the intervention of soybean isoflavones(SI) supplementation on growth performance,intestinal mucosal oxidation-related indicators,immune factors,and co-oxidation and immune-related factors in neonatal piglets,and to explore the effects of oxidative stress on growth and intestinal immune response in neonatal piglets.The neonatal piglets(n=96,4 day old) were selected and divided into four groups.They were fed 5% fresh fish oil(FFO)(group Ⅰ),5% FFO+50 mg/kg SI(group Ⅱ),5% OFO(group Ⅲ),OFO+50 mg/kg SI(group Ⅳ).After 21 d of feeding,two piglets per replicate were slaughtered,and small intestines were sampled.The results were showed as follows: at the end of 21 d,body weight of the neonatal piglets in group Ⅲ was significantly decreased compared with that in group Ⅱ(P0.05).From 1 to 21 d,daily weight gain of the neonatal piglets in group Ⅲ was significantly lower than that in group Ⅰ and group Ⅱ(P0.05).Compared with group Ⅰ and group Ⅱ,the content of malondialdehyde(MDA) in the intestinal mucosa of piglets in group Ⅲ was increased by 90.89%(P0.05) and 73.28%(P0.05),respectively.SI supplementation significantly improved the activity of total superoxide dismutase(T-SOD) in intestinal mucosa(P0.05).The OFO and SI produced a significant interaction effect on the activity of total anti-oxidation capability(T-AOC) in intestinal mucosa(P0.01).The content of interleukin-2(IL-2) in group Ⅲ was significantly higher than the that in group Ⅰ(P0.01) and the contents of interleukin-8(IL-8)(P0.05) and interleukin-10(IL-10)(P0.01) in group Ⅲ were significantly lower than those in group Ⅰ(P0.05),respectively.The OFO significantly increased the activity of inducible nitric oxide synthase(iNOS)(P0.05) and the content of nuclear factor-κB(NF-κB)(P0.01).Collectively,these results suggest that dietary OFO significantly reduces the antioxidant capacity and causes intestinal inflammation in piglets(4 to 24 d old).SI supplementation can alleviate intestinal damage of neonatal piglets.
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