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《Chinese Journal of Control of Endemic Disenaces》 2000-04
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A study on the PCR about primers of 31KD_a protein gene for B.abortus

Li Lanyu,Qiu HaiYin, Shang DaQiu (Institute of Epidemilogy and Microbiology, Chinese Academy Preventwe Medicine, Beijing, 102206)  
Objective: A study on the specificity and sensitivity about PCR of primers of 31KDa protein gene for B.abortus. Methods: DNA were extracted with boilling and phenol from 6 species Brucella,Y.enteroco- litica O3 ed O9,E.coli O157:H7 and S typhimurium strains. Results:the DNA of 6 species Brucella strains were amplified by the primers B4 and B5 of 31KDa, but not for the DNA of other strains. The amplification reaction associated not with extractive methods.A sensitivity of the PCR which examined Brucella DNA is 0.95pg/ μl.Conclusio:The PCR of B4 and B5 primers be of good specificity and sensitivity.
【CateGory Index】: R378.5
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