Effect of anisodamine on changes of blood-brain barrier permeability following brain injury in rabbits
Xu Ruxiang ,Yi Shengyu , Wang Boyun Department of Neurosury, Zhujing Hospital Department of Neurosurgery, Xijin Hospital Fourth Military Medical University, Xian (710032)
The changes of blood-brain barrier ( BBB ) permeability following brain injury were studied quantitatively by using colloidal gold ( CG ) particles of various sizes as tracers in 57 rabbits. In addition,water content in brain tissues was determined. The brain-injured rabbits received intravenous injection of anisodamine in a dose of 0.3 mg/kg BW at 5 min or 3 h after brain injury, and the effect of anisodamine on alterations of BBB permeability and water content of the brain tissue after brain injury was investigated. The results indicated that an increase of BBB permeability began at 30 min after injury with only a few of 5 nm CG tracers present in the endocytic pits and endothelial microvilli,and there were 10 nm CG tracers passing through the BBB at 3 h after brain injury. The increase of BBB permeability reached its peak at 6 h after brain injury. More 15 nm CG tracers penetrated the BBB by way of pinocytotic vesicle transportation and opening of tight junctions between endothelial cells. The water content in the brain tissue increased, which was closely correlated to the above-mentioned BBB permeability. Anisodamine can decrease the BBB permeability markedly, which is of advantages to the treatment of traumatic brain edema.