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Changes of intestinal permeability and histologies of intestinal mucosa in acute necrotizing pancreatitis in dogs

Wu Chengtang; Li Zhanliang; Xiong Dexin(Department of General Surgery, Nanfang Hospital, First Military Medical University, Guangzhou, 510515; Department of GeneralSurgery, The 304th Hospital of PLA, Beijing, 100037)  
Objective To observe the changes in intestinal permeability and pathological changes of the intestinal mucosa in acutenecrotizing pancreatitis (ANP). Methods Fifteen dogs were randomly divided into ANP group (n=8) and control group (n=7). ANPmodel was induced by injection of 0.5 ml/kg 5% sodium taurocholate with 3 000 U/kg trypsin into the pancreatic duct. Gaschromatography was applied to determine the levels of lactulose and mannitol in the urine. All the animals were sacrificed on theseventh postoperative day, and the tissue of the lieal mucosa was collected for histological and ultrastructural studies. Results TheL/M (lactulose/mannitol) ratio levels were 2-10 times higher than that of the controls. The epithelial villi of the mucosa exfoliated,the microvilli and the area of the villi was reduced. Diamine oxidase activities were also decreased. Blood levels oflipopolysaccharide are increased 1 -2 times. The translocation rate of bacteria was 100%. Conclusion ANP induced impairment of thegut barrier function, which led to LPS and bacterium translocation; Determining the changes of L/M in the urine can reflect the earlyinjury of intestinal mucosa in ANP.
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