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Biological effects of basic fibroblast growth factor on bone marrow stromal cells

ZHENG Lei WANG Qian WEI Kuan-hai Pei Guo-xian (Department of Orthopedics and Traumatology. Clinical Laboratory Nanfang Hospital, First Military Medical University. Guangzhou 510515. China)  
objective To investigate the biological effects of basic fibroblast growth factor(hFGF) on the adhesion, proliferation and differentiation of bone marrow stromal cells(BMSC) Methods BMSCs were subjected to the treatment with different concentrations of bFGF. after which the attached cells were estimated with stereological method. cell proliferation measured with methylthiazol tetrazolium (MTT) method and cell differentiation with alkaline phosphatase (ALP) measurement kit Results 10 ng/ml bFGF can promote cell adhension significantly. while 200 ng/ml bFGF inhibited cell adhesion. we also found that 100 ng/ml bFG F could significantly promote cell proliferation, and cellular ALP level. however. was reduced to the lowest of all groups Conclusion bFGF may have complicated and multiple biological effects on BMSC. regulating cell adhension, proliferation and differentiation Its effects are concentration dependent
【Fund】: 国家自然科学基金!(39970743)
【CateGory Index】: R318.01
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