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《Journal of First Military Medical University》 2002-11
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Atomic force microscopic observation of the neurons membrane in primary culture after treatment with N-Methyl-D-Asph

YU Yi-gang 1 , XU Ru-xiang 1 , KE Yi-quan 1 , JIANG Xiao-dan 1 , LIU Shao-jun 2 , YANG Yan-lian 31 Department of Neurosurgery, Zhujiang Hospital, First Military Medical Univer sity, Guangzhou 510282, China; 2 Neurobiology Laboratory, Institute of Basic Medical Science, Academy of Mili tary Medical Sciences, Beijing 100850, China; 3 Nanochemistry Laboratory, College of Chemistry and Molecular Engineering, Ce nter for Nanoscale Science and Technology, Peking University, Beijing 100871, China  
Objective To observe the changes in three-dimensional morpholog y of the membrane of the cortex neurons in pri-mary culture in response to treatm ent with N-Methyl-D-Asp (NMDA) and MK-801 using atomic force microscope (AFM). Methods Following NMDA treatment, the changes in the membrane of the neurons fix ed by glutaraldehyhyde were examined, and the protective effect of MK-801 on th e neurons was also observed using an ATM at the resolution of 0.01-0.1 nm. Resul ts Normal neurons presented smooth membrane surface with regular undulation and densely but well arrayed protein granules. In response to NMDA treatment, the n eurons were disrupted, falling into small pieces, and their membrane appeared di scontinu-ous. MK-801 treatment increased the folds of the membrane that showed r ough edges, with membrane undulation only sec-ondary to normal condition. Conclu sions AFM, with the merits of high resolution and easy sample preparation, can f inely dis-play the three-dimensional morphology of the surface of the neurons, w hich, after NMDA treatment, becomes disintegrated with increased bulk of the pr otein granules, deepened caving of the lipids, and rougher membrane surface.
【Fund】: 国家自然科学基金(30170961);; 全军医药卫生科研基金(01Z054)
【CateGory Index】: R329.24
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