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《Journal of First Military Medical University》 2003-06
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Role of antisense oligodeoxynucleotide of inducible nitric oxide synthase in the apoptosis and p-p38 MAPK signal transduction in rat neurons after spinal cord injury

ZHANG Xin-yu 1 , ZHOU Chu-song 1 , JIN An-min 1 , GUO Ai-lin 2 , TIAN Jing 11 Department of Orthopaedics, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China; 2 Sun Yat-sen Medical University, Guangzhou 510089, China  
Objective To study the role of antisense oligodeoxynucleotides (ASODN) of inducible nitric oxide synthase (iNOS) on the apoptosis and p-p38 mitogen-activated protein kinase (p-p38 MAPK) signal transduction of the neurons after spinal cord injury (SCI). Methods Antisense and non-sense oligodeoxynucleotides of iNOS were designed and synthesized, and injected into the subarachnoid space 12 h before the compressive injury was given to the rat spinal cord. Reverse transcription-PCR and flow-cytometry were used to examine the iNOS mRNA expression and the apoptosis of the neurons within the injured spinal cord. The changes of p-p38 MAPK signal transduction pathway were detected by Western blotting. Results Obvious increase in iNOS expression and up-regulated p-p38 MAPK were detected after SCI, and neuronal apoptosis was observed in the injured spinal cord. ASODN-iNOS treatment resulted in decreased expression levels of iNOS mRNA and p-p38 MAPK, and the neuronal apoptosis was alleviated. Conclusion ASODN- iNOS inhibits iNOS expression and neuronal apoptosis following SCI which may be related to the p-p38 MAPK signal transduction pathway.
【Fund】: 国家自然科学基金(39800166)~~
【CateGory Index】: R651.2
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