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《Journal of First Military Medical University》 2004-10
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Bioinformatic analysis of Vibrio parahaemolyticus thermolabile hemolysin gene

LI Zhi-Feng, NIE Jun, DAI Ying-Chun, LI Jian-dong, CHEN Qing, YU Shou-yiDepartment of Epidemiology, Southern Medical University, Guangzhou 510515, China  
Objective To carry out bioinformatic analysis of Vibrio parahaemolyticus thermolabile hemolysin gene(tlh) obtained by PCR amplification. Methods The tlh gene amplified by PCR was cloned into the vector pET32a+ and sequenced, followed by analysis of the biological information by with presenting the sequences to the websites of bioinformatics on the Internet. Results and conclusions The sequenced tlh gene (named tlh14-90) was entered into GenBank with the accession number of AY289609. Tlh14-90 has a length of 1 257 bp with both start and stop codons, having 99% homology with the tlh gene of WP1. Tlh14-90 is predicted to encode a protein containing 418 amino acids (named TLH14-90, with the molecular formula of C2131H3184N548O649S16, molecular weight of 47 392.9, and the theoretical PI of 4.92). This protein consists of 4 Cys and the contents of Ala, Leu and Asn are 11.0%, 7.4% and 7.2%, respectively, having a hydrophobic parameter of -34 calculated using κ-D method. Predicted as a α/□protein for its secondary structure, TLH14-90 has not been identified for its tertiary structure.
【Fund】: 全军十五指令性课题(01L050)~~
【CateGory Index】: R378
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