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《Journal of Southern Medical University》 2007-05
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Polymorphism of 12 Y-STR loci in Guangzhou Han population and its application in forensic medicine

LIU Chao1, 2, CHEN Ling1, CHEN Xiao-hui2, LIU Chang-hui2, WANG Hui-jun1 1Institute of Forensic Medicine, Southern Medical University, Guangzhou 510515, China; 2Guangzhou Criminal Science and Technology Institute, Guangzhou 510030, China  
Objective To investigate the allele frequency distribution, gene diversity and haplotype diversity of 12 Y-specific short tandem repeats (STR) in Guangzhou Han population and evaluate their forensic application. Methods Twelve Y-STR loci in 401 unrelated male Han individuals in Guangzhou were amplified with PowerPlex Y System, and the PCR products were detected with 3100 Genetic Analyzer. Results The data of allele distribution and gene diversity of the 12 Y-STR loci were obtained from these individuals. A total of 398 haplotypes were observed and the overall haplotype diversity for the 12 Y-STR loci was 0.99997. The DNA samples of 13 different species of animals were amplified and no specific products were observed. Different tissues from the same individual exhibited the same Y-STR haplotype. Conclusion The 12 Y-STR loci exhibit high genetic polymorphism and are of important value in understanding of the human origin, individual identification for mixed male/female DNA samples and paternity identification.
【Fund】: 国家自然科学基金(30400519);; 广州市重大科技攻关项目(2005Z1-E0051)~~
【CateGory Index】: D919
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