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《Journal of Southern Medical University》 2007-12
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Protective effect of ketamine against acute rat lung injury induced by liopolysaccharide and its mechanism

WANG Xu-dong1, LU Ya-li1, LAI Ren-chun1, LI Yan-ping2, HUANG Wan1, XU Mei1 1Department of Anesthesiology, Oncology Center, Sun Yat-sen University, Guangzhou 510060, China; 2Department of Anesthesiology, Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120, China  
Objective To investigate the protective effects of different concentrations of ketamine against acute lung injury induced by lipopolysaccharide (LPS) in rats and its mechanism. Methods Forty-eight male Wistar rats were randomized into 4 equal groups, namely the control group, LPS group, ketamine groupⅠ(5 mg/kg), and ketamine group Ⅱ (10 mg/kg). The neutrophil count, protein contents in the bronchoalveolar lavage fluid (BALF) and the wet/dry lung weight ratio were measured 4 h after LPS injection. TNF-α, IL-8, NO, iNOS and NF-κB were also measured in the lung tissues. Results In LPS group, the neutrophil count, protein contents in BALF, the wet/dry lung weight ratio and the levels of tumor necrosis factor-α (TNF-α), interleukin-8 (IL-8), and NO were all significantly increased compared with the control group (P0.01). The mRNA expression of iNOS and the protein expression of NF-κB were also increased in LPS groups. Ketamine treatment attenuated the increase in wet/dry lung weight ratio, neutrophil count, and protein contents in BALF in a dose-dependent manner. Ketamine also dose-dependently inhibited the production of TNF-α, IL-8 , and NO and lowered iNOS mRNA and NF-κB protein expression. Conclusion Ketamine can offer protection against LPS-induced acute lung injury in rats by inhibiting the expression of NF-κB and attenuating the production of the inflammatory cytokines.
【Fund】: 广东省科技计划项目(2005B30301001);; 广东省医学科研项目(A2005249)
【CateGory Index】: R563.8
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