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《Journal of Southern Medical University》 2008-09
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Establishment of a nude mouse model of nasopharyngeal carcinoma lymph node metastasis and screening of the metastasis-related signature genes

LENG Lei,LIU Teng-fei,HUANG Zhong-xi,XIE Wei-bing,YAO Kai-tai Cancer Institute,Southern Medical University,Guangzhou 510515,China  
Objective To establish a nude mouse model of nasopharyngeal carcinoma(NPC) lymph node metastasis and screen the signature genes associated with the metastasis.Methods The NPC 5-8F-EGFP cells were inoculated into nude mice,from which a 5-8F-LN cell line with lymph node metastasis potential was obtained.The lymphatic metastasis-related signature genes of breast cancer and head and neck squamous cell carcinoma were screened by data mining method.Results The NPC cell lines 5-8F and 6-10B showed 307 differentially expressed genes by microarray analysis,from which 20 overlapping genes were identified,and 3 overexpressed genes were found with probable metastasis potential,namely the ADM,IRF1,and CAV1 genes.Quantitative RT-PCR validated the data mining results in the 5-8F-EGFP,6-10B-EGFP,NP69,and 5-8F-LN cell lines.The 3 NPC cell lines 5-8F-EGFP,6-10B-EGFP and 5-8F-LN showed significantly higher expressions of IRF1 than NP69 cells(P=0.008,0.022,and 0.006,respectively.The expression level of CAV1 in 5-8F-EGFP cells was significantly higher than that in 6-10B-EGFP cells(P=0.014),but ADM expression showed no significant difference between the 4 cell lines.Conclusions IRF1 may play an important role in the progression of NPC.The overexpression of CAV1 in 5-8F-EGFP cells can be associated with the high metastatic potential of the cells.
【Fund】: 国家自然科学基金委员会-广东省人民政府联合基金(U0732006)
【CateGory Index】: R739.63
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