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《Journal of Southern Medical University》 2008-10
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Qufeng Tongluo decoction inhibits lipopolysaccharide-induced rat mesangial cell proliferation in vitro by suppressing NF-κB activation and reducing TGF-β1 and IL-6 mRNA expressions

WANG Zhu, SUN Wan-sen, WANG Juan, MA Qiao-ya, ZHANG Xiao-qiang, WU Xi-li Department of Integrated Traditional Chinese and Western Medicine, Second Hospital Affiliated to Xi'an Jiaotong University College of Medicine, Xi'an 710004, China  
Objective To investigate the effect of Qufeng Tongluo(QFTL) decoction on lipopolysaccharide(LPS)-induced rat mesangial cell proliferation and explore the possible mechanisms underlying the therapeutic effects.Methods Thirty-two rats were randomized into normal control, glomerulonephritis model, QFTL treatment and positive control groups, and serum samples were obtained from these groups.Rat mesangial cells with or without LPS exposure were treated with the sera, and the expression of nuclear factor-κB(NF-κB ) was detected using electrophoretic mobility shift assay(EMSA), and the expressions of transforming growth factor-β1(TGF-β1) and interleukin-6(IL-6) mRNAs were detected with RT-PCR.Results QFTL decoction inhibited the activation of NF-κB in LPS-stimulated rat mesangial cells stimulated by LSP, and lowered the expressions of TGF-β1 and IL-6 mRNA.Conclusion QFTL decoction can inhibit LPS-induced rat mesangial cell proliferation by decreasing the expression of TGF-β1 and IL-6 mRNA as a result of suppression NF-κB activation.
【Fund】: 陕西省科技计划项目基金(2004K16-G9);; 陕西省中医管理局项目基金(2005028)
【CateGory Index】: R285.5
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