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《Journal of Southern Medical University》 2009-01
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Effect of recombinant caspase-3 gene transfection on CNE2 cell apoptosis

WANG xiao-xia1,2,YAO xiao-bao1, JI xian-sheng2, ZHU hong-liang1 1Department of Otolaryngology, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710061, China;2Department of Otolaryngology, 451 Hospital of PLA, Xi'an 710054, China  
Objective To investigate the apoptosis-inducing effect of caspase-3 on human nasopharyngeal carcinoma cells(CNE2).Methods Recombinant caspase-3 was subcloned into the eukaryotic expression vector PEGFP-C1 containing the reporter gene using DNA recombinant technique.CNE2 cells were transfected with the recombinant caspase-3 gene via lipofectamine 2000 and the expression of caspase-3 mRNA was detected by reverse transcription-polymerase chain reaction.The cell morphological changes were observed under fluorescence microscope and electron microscope and the cell survival rate after the transfection was assessed by MTT assay.Results Transfection with the recombinant caspase-3 gene induced significant apoptosis in CNE2 cells, which exhibited obvious morphological changes typical of apoptotic cells.Conclusion The recombinant caspase-3 gene can inhibit the growth and effectively induce apoptosis of CNE2 cells.
【CateGory Index】: Q789
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