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《Journal of Southern Medical University》 2009-04
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Biocompatibility between human adipose-derived mesenchymal stem cells and porcine bone scaffolds

ZHANG Xue-quan, YUAG Lin, YANG Lin-lin, JANG Xue-mei, YANG Chun, YU Lei, DAI Jing-xing Department of Human Anatomy, South Medical University, Guangzhou 510510, China  
Objective To study the compatibility between human adipose-derived mensenchymal stem cells and porcine bone scaffolds. Methods Porcine bone tissues were co-cultured with adipose-derived mesenchymal stem cells, and the complex was observed under scanning electron microscope. The viability and alkaline phosphatase (ALP) activity of the cells were examined with the cells co-cultured with human bone scaffold as the control. Results At 4 and 10 days after the co-culture, the adipose-derived mesenchymal stem cells were observed to extend pseudopodia to adhere to the two scaffold materials. MTT assay showed that the cell proliferation on both of the materials increased with time, and the two cell complexes exhibited similar pattern of changes in ALP activity. Conclusion As the seed cells, human adipose-derived mesenchymal stem cells exhibit good comparability with porcine bone scaffold, suggesting their potential of constructing tissue-engineered bone graft.
【Fund】: 国家重点基础研究发展计划973计划课题(2007CB512705)
【CateGory Index】: R318.08
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 TIAN Jiajun, ZHENG Hua, CHEN Dunchun, WANG Dingguo. Biomedical Material Inspect Center of Sichuan Province, Chengdu 610041;Biological Evaluation of Biological Derivation Bone Tissue Engineering Scaffold[J];Beijing Biomedical Engineering;2003-04
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【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 WANG Zhong, GAO Yi, WANG Yan, PAN Ming-xin. Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China;Isolation, cultivation and biological identification of human bone marrow mesenchymal stem cells[J];Chinese Journal of Neuromedicine;2006-10
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