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《Journal of Southern Medical University》 2010-10
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Immunophenotypic comparison of adipose-derived mesenchymal stem cells from the greater omentums and subcutaneous adipose tissues of rats

LI Dong-fei1,YANG Chun1,LI Zhen1,YUAN Ling-ling2,DAI Jing-xing1,YUAN Lin1 Department of Human Anatomy1,School of Basic Medical Sciences2,Southern Medical University,Guangzhou 510515,China  
Objective To compare the adipose-derived mesenchymal stem cells(ADMSCs) isolated from the greater omentums and subcutaneous adipose tissues of rats for their characteristics in cell morphology,growth kinetics and immunophenotypes.Methods ADMSCs were isolated from the greater omentums and inguinal fat pads of 6 SD rats and cultured in vitro.The morphologies of the ADMSCs were observed using phase-contrast microscopy,and theirgrowth curves were generated and the doubling times determined.The phenotypic marker profiles including CD11b,CD29,CD45,CD49d,CD90 and CD106 of the ADMSCs in the fourth passage were determined using flow cytometry.Results The ADMSCs harvested from the greateromentums and inguinal fat pads showed almost identical morphologies.The growth curves and the mean doubling time of the ADMSCs from the two different sources showed no obvious difference.With similar positivity rates for CD11b,CD29,CD106 and CD90,the two ADMSCs exhibited different expression rates of CD45 and CD49d.Conclusions The immunophenotypic characteristics of the ADMSCs from the greater omentums and subcutaneous adipose tissues are not totally identical.
【Fund】: 国家重点基础研究计划(973计划)(2007CB512705);; 国家自然基金青年面上(30801464)~~
【CateGory Index】: R329
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