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《Journal of Southern Medical University》 2012-12
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Effects of Qufengtongluo Recipe on expressions of cell cycle regulatory proteins in rat mesangial cells

WU Xili1,SUN Wansen1,YE Bingyu1,AN Peng1,SHI Xingmin2,FU Rongguo1,WANG Zhu1,QIAO Chenglin1 1Department of Integrated Traditional Chinese and Western Medicine,Second Affiliated Hospital,Xi'an Jiaotong University College of Medicine,Xi'an 710004,China;2Xi'an Jiaotong University College of Medicine,Xi'an 710061,China  
Objective To observe the effects of Qufengtongluo Recipe(QFTLR) on the expressions of cell cycle regulatory proteins in rat mesangial cells(MCs) in vitro and investigate the mechanism by which QFTLR inhibits MC proliferation.Methods Using the methods of serum pharmacology,we studied the expressions of cell cycle regulatory proteins in rat MCs treated with QFTLR by laser scanning confocal microscope and immunohistochemistry.Results Compared with the normal control cells,the cells challenged with lipopolysaccharide(LPS) showed significantly enhanced expressions of cyclin D1,CDK2,and P21(P0.01) and obviously lowered protein expression of P27(P0.01).Treatment of the LPS-challenged cells with QFTLR and benazepril both resulted in significantly attenuated expressions of cyclin D1,CDK2,and P21 and obvious increase of P27 expression(P0.05 or P0.01),but QFTLR produced stronger effects than benazepril in regulating of cyclinD1,P21 and P27 protein expressions(P0.05 or P0.01).Conclusion QFTLR inhibits rat MC proliferation in vitro possibly by down-regulating the cellular expressions of cyclin D1,CDK2,and P21 and up-regulating the expression of P27 protein.
【Fund】: 国家自然科学基金(30801504 30901926 81100530 81070590);; 陕西省科技计划项目(2012K19-04-01)~~
【CateGory Index】: R285.5
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