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Effect of plant polysaccharides on phosphatidylinositol turnover of the plasma membrane of Sarcoma S_180 cell

Huang Tianyou, Tong Li,Liang Mou,Wu Bo,Liang Nianci 1. The Fist Military Medical Univereity of PLA. Guangzhou 510515 2.Guangdong Medical College, Zhanjiang 524023  
Mice sarcoma S_180 cell membranes,[γ-32]ATP and plant polysaccharide(PPS,ASPS,TF orlentinan)were incubated together for 5 min at 30 ℃,Phosphatidylinositides were extracted and isolated,and radioactivity was measured with liquid scintillation counter.The results showed that PPS and ASPS,especially the latter,could inhibit the phosphatidyinostol turnover obviously,while TF and lentinan hadno such effect Combining with our previous findings that PPS and ASPS could inhibit the proliferation ofS_180 cell significantly while TF and lentinan had no such effect,we conisider that the antitumor mechanismof plant polysaccharides(PPS and ASPS)may be related to the inhibition of phosphatidyinositol turnover.
【Fund】: 1992年度全军青年科学基金
【CateGory Index】: Q73
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