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Site directed mutagenesis of human brain derived neurotrophic factor gene

Li Jun 1,Shu Siyun 2,Bao Xinmin 2,Qi Zhengwu 3 1Department of Biochemitry,First Military Medical University;Guang zhou, 510515; 2Department of Neurobiology,Zhujiang Hospital,Guang zhou,510282; 3National Molecular Biology Key Lab  
Human brain derived neurotrophic factor gene was used as template for oligonucleotide based site directed mutagenesis and PCR.Mutated genes were confirmed by nucleotide sequence analysis.This lays the foundation of structure and function studies of brain derived neurotrophic factor.
【CateGory Index】: Q42,Q343.13
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